Oncostatin m impairs brown adipose tissue thermogenic function and the browning of subcutaneous white adipose tissue.
Por:
Sánchez-Infantes D, Cereijo R, Peyrou M, Piquer-Garcia I, Stephens JM and Villarroya-Gombau F
Publicada:
1 ene 2017
Ahead of Print:
5 oct 2016
Resumen:
OBJECTIVE: Since oncostatin m (OSM) is elevated in adipose tissue in conditions of obesity and type 2 diabetes in mice and humans, the aim of this study was to determine whether this cytokine plays a crucial role in the impairment of brown adipose tissue (BAT) activity and browning capacity that has been observed in people with obesity. METHODS: C57BL/6J mice rendered obese by high-fat diet, their lean controls, and C57BL/6J mice fed a standard diet and implanted subcutaneously with a mini pump through a surgical procedure to deliver OSM or placebo were used. Preadipocytes or fully differentiated brown adipocytes were treated with OSM or vehicle with or without norepinephrine before harvesting. RNA was extracted and processed for qPCR analysis. Media from mature adipocytes was also collected to measure glycerol levels. RESULTS: Studies demonstrated that OSM gene expression was increased in BAT of mice fed a high-fat diet. In addition, exogenous OSM impaired BAT activity and the browning capacity of white adipose tissue in vitro and in vivo. CONCLUSIONS: Overall, the results reveal a negative role for OSM on BAT and on the browning of white adipose tissue. Therefore, further studies are necessary to demonstrate whether OSM inhibition is a potential treatment for metabolic disorders.
Filiaciones:
Sánchez-Infantes D:
Department of Endocrinology and Nutrition, Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol, Barcelona, Spain.
Cereijo R:
Department of Biochemistry and Molecular Biology, and Institute of Biomedicine, University of Barcelona, Barcelona, Spain
Centro de Investigación Biomédica en Red Fisiopatología de la Obesidad y Nutrición (CIBEROBN), ISCIII, Madrid, Spain
Institut de Recerca Pediàtrica Hospital Sant Joan de Déu (IRP-HSJD) University of Barcelona, Barcelona, Spain
Peyrou M:
Department of Biochemistry and Molecular Biology, and Institute of Biomedicine, University of Barcelona, Barcelona, Spain
Centro de Investigación Biomédica en Red Fisiopatología de la Obesidad y Nutrición (CIBEROBN), ISCIII, Madrid, Spain
Institut de Recerca Pediàtrica Hospital Sant Joan de Déu (IRP-HSJD) University of Barcelona, Barcelona, Spain
Piquer-Garcia I:
Department of Endocrinology and Nutrition, Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol, Barcelona, Spain
Stephens JM:
Department of Biological Science, Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, Louisiana, USA
Villarroya-Gombau F:
Department of Biochemistry and Molecular Biology, and Institute of Biomedicine, University of Barcelona, Barcelona, Spain
Centro de Investigación Biomédica en Red Fisiopatología de la Obesidad y Nutrición (CIBEROBN), ISCIII, Madrid, Spain
Institut de Recerca Pediàtrica Hospital Sant Joan de Déu (IRP-HSJD) University of Barcelona, Barcelona, Spain
Open Access
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