Heparinization of Beta Tricalcium Phosphate: Osteo-immunomodulatory Effects.
Por:
Díez Escudero A, Español-Pons M, Bonany M, Lu X, Persson C and Ginebra MP
Publicada:
1 mar 2018
Ahead of Print:
21 dic 2017
Resumen:
Immune cells play a vital role in regulating bone dynamics. This has boosted the interest in developing biomaterials that can modulate both the immune and skeletal systems. In this study, calcium phosphates discs (i.e., beta-tricalcium phosphate, ß-TCP) are functionalized with heparin to investigate the effects on immune and stem cell responses. The results show that the functionalized surfaces downregulate the release of hydrogen peroxide and proinflammatory cytokines (tumor necrosis factor alpha and interleukin 1 beta) from human monocytes and neutrophils, compared to nonfunctionalized discs. The macrophages show both elongated and round shapes on the two ceramic substrates, but the morphology of cells on heparinized ß-TCP tends toward a higher elongation after 72 h. The heparinized substrates support rat mesenchymal stem cell (MSC) adhesion and proliferation, and anticipate the differentiation toward the osteoblastic lineage as compared to ß-TCP and control. The coupling between the inflammatory response and osteogenesis is assessed by culturing MSCs with the macrophage supernatants. The downregulation of inflammation in contact with the heparinized substrates induces higher expression of bone-related markers by MSCs.
Filiaciones:
Díez Escudero A:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgical Engineering, Universitat Politecnica de Catalunya (UPC), EEBE, Av. Eduard Maristany 10-14, 08019, Barcelona, Spain
Barcelona Research Centre for Multiscale Science and Engineering, Universitat Politecnica de Catalunya (UPC), EEBE, Av. Eduard Maristany 10-14, 08019, Barcelona, Spain
Español-Pons M:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgical Engineering, Universitat Politecnica de Catalunya (UPC), EEBE, Av. Eduard Maristany 10-14, 08019, Barcelona, Spain
Barcelona Research Centre for Multiscale Science and Engineering, Universitat Politecnica de Catalunya (UPC), EEBE, Av. Eduard Maristany 10-14, 08019, Barcelona, Spain
Bonany M:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgical Engineering, Universitat Politecnica de Catalunya (UPC), EEBE, Av. Eduard Maristany 10-14, 08019, Barcelona, Spain
Barcelona Research Centre for Multiscale Science and Engineering, Universitat Politecnica de Catalunya (UPC), EEBE, Av. Eduard Maristany 10-14, 08019, Barcelona, Spain
Lu X:
Materials in Medicine Group, Division of Applied Materials Science, Department of Engineering Science, Uppsala University, Lagerhyddsy. 1, 751 21, Uppsala, Sweden
Persson C:
Materials in Medicine Group, Division of Applied Materials Science, Department of Engineering Science, Uppsala University, Lagerhyddsy. 1, 751 21, Uppsala, Sweden
Ginebra MP:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgical Engineering, Universitat Politecnica de Catalunya (UPC), EEBE, Av. Eduard Maristany 10-14, 08019, Barcelona, Spain
Barcelona Research Centre for Multiscale Science and Engineering, Universitat Politecnica de Catalunya (UPC), EEBE, Av. Eduard Maristany 10-14, 08019, Barcelona, Spain
Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology, C/ Baldiri Reixac 10-12, 08028, Barcelona, Spain
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