Congenital cytomegalovirus, parvovirus and enterovirus infection in Mozambican newborns at birth: A cross-sectional survey
Por:
Madrid L, Varo R, Maculuve S, Nhampossa T, Munoz-Almagro C, Calderón EJ, Esteva-Afonso C, Carrilho C, Ismail M, Vieites B, Friaza V, Lozano-Dominguez MDC, Menéndez C and Bassat Q
Publicada:
14 mar 2018
Ahead of Print:
14 mar 2018
Resumen:
Background
Congenital cytomegalovirus (cCMV) infection is the most prevalent
congenital infection acquired worldwide, with higher incidence in
developing countries and among HIV-exposed children. Less is known
regarding vertical transmission of parvovirus B19 (B19V) and enterovirus
(EV). We aimed to assess the prevalence of CMV, B19V and EV vertical
transmission and compare results of screening of congenital CMV obtained
from two different specimens in a semirural Mozambican maternity.
Methods
A cross sectional study was conducted among pregnant mothers attending
Manhica District Hospital upon delivery. Information on maternal risk
factors was ascertained. Dried umbilical cord (DUC) samples were
collected in filter paper for CMV, B19V and EV detection by real-ime
polymerase chain reaction (RT-PCR), and nasopharyngeal aspirates (NPA)
to test for CMV by RT-PCR. Maternal blood samples and placental biopsy
samples were also obtained to investigate CMV maternal serology, HIV
status and immunopathology.
Results
From September 2014 to January 2015, 118 mothers/newborn pairs were
recruited. Prevalence of maternal HIV infection was 31.4% (37/118). CMV
RT-PCR was positive in 3/115 (2.6%) of DUC samples and in 3/96 (6.3%) of
NPA samples obtained from neonates. The concordance of the RT-PCR assay
through DUC with their correspondent NPA sample was moderate (Kappa =
0.42 and p < 0.001. No differences on cCMV prevalence were found among
HIV-exposed and unexposed. All (100%) mothers were seropositive for CMV
IgG. RT-PCR of EV and B19V in DUC were both negative in all screened
cases. No histological specific findings were found in placental
tissues. No risk factors associated to vertical transmission of these
viral infections were found.
Conclusions
This study indicates the significant occurrence of vertical transmission
of CMV in southern Mozambique. Larger studies are needed to evaluate the
true burden, clinical relevance and consequences of congenital
infections with such pathogens in resource-constrained settings.
Filiaciones:
Madrid L:
Centro de Investigação em Saúde de Manhiça (CISM), Maputo, Mozambique
ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clinic - Universitat de Barcelona, Barcelona, Spain
:
Centro de Investigação em Saúde de Manhiça (CISM), Maputo, Mozambique
ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clinic - Universitat de Barcelona, Barcelona, Spain
Maculuve S:
Centro de Investigação em Saúde de Manhiça (CISM), Maputo, Mozambique
Nhampossa T:
Centro de Investigação em Saúde de Manhiça (CISM), Maputo, Mozambique
Munoz-Almagro C:
Molecular Microbiology Department, Institut de Recerca Sant Joan de Déu, University Hospital Sant Joan de Déu, Barcelona, Spain
CIBER de Epidemiología y Salud Pública CIBERESP, Instituto de Salud Carlos III, Madrid, Spain
School of Medicine. Universitat Internacional de Catalunya, Barcelona, Spain
Calderón EJ:
CIBER de Epidemiología y Salud Pública CIBERESP, Instituto de Salud Carlos III, Madrid, Spain
Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Seville, Spain
Esteva-Afonso C:
Molecular Microbiology Department, Institut de Recerca Sant Joan de Déu, University Hospital Sant Joan de Déu, Barcelona, Spain
Carrilho C:
Department of Pathology, Maputo Central Hospital, Maputo, Mozambique
Faculty of Medicine, Eduardo Mondlane University, Maputo, Mozambique
Ismail M:
Department of Pathology, Maputo Central Hospital, Maputo, Mozambique
Faculty of Medicine, Eduardo Mondlane University, Maputo, Mozambique
Vieites B:
Department of Microbiology, Hospital Universitario Virgen del Rocío, Seville, Spain
Friaza V:
CIBER de Epidemiología y Salud Pública CIBERESP, Instituto de Salud Carlos III, Madrid, Spain
Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Seville, Spain
Lozano-Dominguez MDC:
Department of Pathology, Hospital Universitario Virgen del Rocío, Seville, Spain
Menéndez C:
Centro de Investigação em Saúde de Manhiça (CISM), Maputo, Mozambique
ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clinic - Universitat de Barcelona, Barcelona, Spain
CIBER de Epidemiología y Salud Pública CIBERESP, Instituto de Salud Carlos III, Madrid, Spain
Bassat Q:
Centro de Investigação em Saúde de Manhiça (CISM), Maputo, Mozambique
ISGlobal, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clinic - Universitat de Barcelona, Barcelona, Spain
ICREA, Barcelona, Spain
Pediatric Infectious Diseases Unit, Pediatrics Department, Hospital Sant Joan de Déu (University of Barcelona), Barcelona, Spain
Green Submitted, gold
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