Expression of the nucleoside transporters hENT1 (SLC29) and hCNT1 (SLC28) in pediatric acute myeloid leukemia.
Por:
Jaramillo AC, Hubeek I, Broekhuizen R, Pastor-Anglada M, Kaspers GJL, Jansen G, Cloos J and Peters GJ
Publicada:
20 abr 2020
Ahead of Print:
20 abr 2020
Resumen:
Cellular uptake of clinically important deoxynucleoside analogs is mediated by nucleoside transporters including the human equilibrative nucleoside transporter 1 (hENT1) and the concentrative nucleoside transporter-1 (hCNT1). These transporters are responsible for influx of cytarabine and reduced hENT1 expression is a major resistance mechanism in acute myeloid leukemia. We determined hENT1 and hCNT1 protein expression by immunocytochemistry in 50 diagnostic pediatric acute myeloid leukemia patient samples. All samples expressed hENT1 [9/43 (21%) low; 26/43 (60%) medium and 8/43 (19%) high] and hCNT1 [2/42 (5%) low; 35/42 (83%) medium and 5/42 (12%) high] at the cell membrane and cytoplasm. Statistical analysis showed a non-significant relationship between survival and transporter expression and in vitro drug sensitivity. In conclusion, the nucleoside transporters hENT1 and hCNT1 are broadly expressed in pediatric acute myeloid leukemia at diagnosis.
Filiaciones:
Jaramillo AC:
Depts of Pediatric Hematology, Amsterdam University Medical Centers, Location VUMC, Amsterdam, The Netherlands
Hubeek I:
Clinical Chemistry, Amsterdam University Medical Centers, Location VUMC, Amsterdam, The Netherlands
Broekhuizen R:
Depts of Pediatric Hematology, Amsterdam University Medical Centers, Location VUMC, Amsterdam, The Netherlands
Department of Hematology-Oncology, Pontifical Catholic University of Chile, Santiago, Chile
Pastor-Anglada M:
Department de Bioquímica i Biologia Molecular, Universitat de Barcelona, Barcelona, Spain
Kaspers GJL:
Princess Maxima Center for Pediatric Cancer, Utrecht, and SKION, The Netherlands
Emma's Children's Hospital, Amsterdam UMC, Location VUMC, Pediatric Oncology, Amsterdam, The Netherlands
Jansen G:
Reumatology, Amsterdam University Medical Centers, Location VUMC, Amsterdam, The Netherlands
Cloos J:
Depts of Pediatric Hematology, Amsterdam University Medical Centers, Location VUMC, Amsterdam, The Netherlands
Peters GJ:
Laboratory Medical Oncology, Amsterdam University Medical Centers, Location VUMC, Amsterdam, The Netherlands
Department of Biochemistry, Medical University of Gdansk, Gdansk, Poland
Open Access
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