A Dual Molecular Biointerface Combining RGD and KRSR Sequences Improves Osteoblastic Functions by Synergizing Integrin and Cell-Membrane Proteoglycan Binding.
Por:
Hoyos M, Falgueras-Batlle E, Ginebra MP, Manero JM, Gil-Mur FX and Mas C
Publicada:
21 mar 2019
Ahead of Print:
21 mar 2019
Resumen:
Synergizing integrin and cell-membrane heparan sulfate proteoglycan signaling on biomaterials through peptidic sequences is known to have beneficial effects in the attachment and behavior of osteoblasts; however, controlling the exact amount and ratio of peptides tethered on a surface is challenging. Here, we present a dual molecular-based biointerface combining integrin (RGD) and heparin (KRSR)-binding peptides in a chemically controlled fashion. To this end, a tailor-made synthetic platform (PLATF) was designed and synthesized by solid-phase methodologies. The PLATF and the control linear peptides (RGD or KRSR) were covalently bound to titanium via silanization. Physicochemical characterization by means of contact angle, Raman spectroscopy and XPS proved the successful and stable grafting of the molecules. The biological potential of the biointerfaces was measured with osteoblastic (Saos-2) cells both at short and long incubation periods. Biomolecule grafting (either the PLATF, RGD or KRSR) statistically improved ( p < 0.05) cell attachment, spreading, proliferation and mineralization, compared to control titanium. Moreover, the molecular PLATF biointerface synergistically enhanced mineralization ( p < 0.05) of Saos-2 cells compared to RGD or KRSR alone. These results indicate that dual-function coatings may serve to improve the bioactivity of medical implants by mimicking synergistic receptor binding.
Filiaciones:
Hoyos M:
Biomaterials, Biomechanics and Tissue Engineering Group (BBT), Department of Materials Science and Metallurgical Engineering, Universitat Politècnica de Catalunya (UPC), 08019 Barcelona, Spain.
Barcelona Research Center in Multiscale Science and Engineering, UPC, 08019 Barcelona, Spain.
Falgueras-Batlle E:
Biomaterials, Biomechanics and Tissue Engineering Group (BBT), Department of Materials Science and Metallurgical Engineering, Universitat Politècnica de Catalunya (UPC), 08019 Barcelona, Spain.
Barcelona Research Center in Multiscale Science and Engineering, UPC, 08019 Barcelona, Spain.
Ginebra MP:
Biomaterials, Biomechanics and Tissue Engineering Group (BBT), Department of Materials Science and Metallurgical Engineering, Universitat Politècnica de Catalunya (UPC), 08019 Barcelona, Spain.
Barcelona Research Center in Multiscale Science and Engineering, UPC, 08019 Barcelona, Spain.
Manero JM:
Biomaterials, Biomechanics and Tissue Engineering Group (BBT), Department of Materials Science and Metallurgical Engineering, Universitat Politècnica de Catalunya (UPC), 08019 Barcelona, Spain.
Barcelona Research Center in Multiscale Science and Engineering, UPC, 08019 Barcelona, Spain.
Gil-Mur FX:
Biomaterials, Biomechanics and Tissue Engineering Group (BBT), Department of Materials Science and Metallurgical Engineering, Universitat Politècnica de Catalunya (UPC), 08019 Barcelona, Spain.
Universitat Internacional de Catalunya (UIC), 08195 Sant Cugat del Vallès, Spain.
Mas C:
Biomaterials, Biomechanics and Tissue Engineering Group (BBT), Department of Materials Science and Metallurgical Engineering, Universitat Politècnica de Catalunya (UPC), 08019 Barcelona, Spain.
Barcelona Research Center in Multiscale Science and Engineering, UPC, 08019 Barcelona, Spain.
Open Access
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