Proteomic Profiling of Retinoblastoma-Derived Exosomes Reveals Potential Biomarkers of Vitreous Seeding
Por:
Galardi A, Colletti M, Lavarello C, Paolo VD, Mascio P, Russo I, Cozza R, Romanzo A, Valente P, Vito R, Pascucci L, Peinado H, Carcaboso AM, Petretto A, Locatelli F and Giannatale AD
Publicada:
1 jun 2020
Ahead of Print:
12 jun 2020
Resumen:
Retinoblastoma (RB) is the most common tumor of the eye in early
childhood. Although recent advances in conservative treatment have
greatly improved the visual outcome, local tumor control remains
difficult in the presence of massive vitreous seeding. Traditional
biopsy has long been considered unsafe in RB, due to the risk of
extraocular spread. Thus, the identification of new biomarkers is
crucial to design safer diagnostic and more effective therapeutic
approaches. Exosomes, membrane-derived nanovesicles that are secreted
abundantly by aggressive tumor cells and that can be isolated from
several biological fluids, represent an interesting alternative for the
detection of tumor-associated biomarkers. In this study, we defined the
protein signature of exosomes released by RB tumors (RBT) and vitreous
seeding (RBVS) primary cell lines by high resolution mass spectrometry.
A total of 5666 proteins were identified. Among these, 5223 and 3637
were expressed in exosomes RBT and one RBVS group, respectively. Gene
enrichment analysis of exclusively and differentially expressed proteins
and network analysis identified in RBVS exosomes upregulated proteins
specifically related to invasion and metastasis, such as proteins
involved in extracellular matrix (ECM) remodeling and interaction,
resistance to anoikis and the metabolism/catabolism of glucose and amino
acids.
Filiaciones:
Galardi A:
Department of Pediatric Hematology/Oncology and Cell and Gene Therapy, IRCCS, Ospedale Pediatrico Bambino Gesù, Piazza Sant'Onofrio 4, 00165 Rome, Italy
Colletti M:
Department of Pediatric Hematology/Oncology and Cell and Gene Therapy, IRCCS, Ospedale Pediatrico Bambino Gesù, Piazza Sant'Onofrio 4, 00165 Rome, Italy
Lavarello C:
Core Facilities-Clinical Proteomics and Metabolomics, IRCCS, Istituto Giannina Gaslini, Via Gerolamo Gaslini 5, 16147 Genoa, Italy
Paolo VD:
Department of Pediatric Hematology/Oncology and Cell and Gene Therapy, IRCCS, Ospedale Pediatrico Bambino Gesù, Piazza Sant'Onofrio 4, 00165 Rome, Italy
Mascio P:
Department of Pediatric Hematology/Oncology and Cell and Gene Therapy, IRCCS, Ospedale Pediatrico Bambino Gesù, Piazza Sant'Onofrio 4, 00165 Rome, Italy
Russo I:
Department of Pediatric Hematology/Oncology and Cell and Gene Therapy, IRCCS, Ospedale Pediatrico Bambino Gesù, Piazza Sant'Onofrio 4, 00165 Rome, Italy
Cozza R:
Department of Pediatric Hematology/Oncology and Cell and Gene Therapy, IRCCS, Ospedale Pediatrico Bambino Gesù, Piazza Sant'Onofrio 4, 00165 Rome, Italy
Romanzo A:
Ophtalmology Unit, IRCCS, Ospedale Pediatrico Bambino Gesù, Piazza Sant' Onofrio 4, 00165 Rome, Italy
Valente P:
Ophtalmology Unit, IRCCS, Ospedale Pediatrico Bambino Gesù, Piazza Sant' Onofrio 4, 00165 Rome, Italy
Vito R:
Department of Pathology, IRCCS, Ospedale Pediatrico Bambino Gesù, Piazza di Sant' Onofrio 4, 00165 Rome, Italy
Pascucci L:
Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy
Peinado H:
Microenvironment & Metastasis Group, Molecular Oncology Program, Spanish National Cancer Research Centre (CNIO), C/Melchor Fernández Almagro 3, 28029 Madrid, Spain
Carcaboso AM:
Pediatric Hematology and Oncology, Hospital Sant Joan de Deu, Institut de Recerca Sant Joan de Deu, Barcelona, 08950 Esplugues de Llobregat, Spain
Petretto A:
Core Facilities-Clinical Proteomics and Metabolomics, IRCCS, Istituto Giannina Gaslini, Via Gerolamo Gaslini 5, 16147 Genoa, Italy
Locatelli F:
Department of Pediatric Hematology/Oncology and Cell and Gene Therapy, IRCCS, Ospedale Pediatrico Bambino Gesù, Piazza Sant'Onofrio 4, 00165 Rome, Italy
Department of Ginecology/Obstetrics & Pediatrics, Sapienza University of Rome, 00185 Roma, Italy
Giannatale AD:
Department of Pediatric Hematology/Oncology and Cell and Gene Therapy, IRCCS, Ospedale Pediatrico Bambino Gesù, Piazza Sant'Onofrio 4, 00165 Rome, Italy
Green Submitted, gold, Green Published
|