Positive Effect of Cold Atmospheric Nitrogen Plasma on the Behavior of Mesenchymal Stem Cells Cultured on a Bone Scaffold Containing Iron Oxide-Loaded Silica Nanoparticles Catalyst.
Por:
Przekora A, Audemar M, Pawlat J, Canal-Barnils C, Thomann JS, Labay C, Wojcik M, Kwiatkowski M, Terebun P, Ginalska G, Hermans S and Duday D
Publicada:
3 jul 2020
Ahead of Print:
3 jul 2020
Resumen:
Low-temperature atmospheric pressure plasma was demonstrated to have an ability to generate different reactive oxygen and nitrogen species (RONS), showing wide biological actions. Within this study, mesoporous silica nanoparticles (NPs) and Fe(x)O(y)/NPs catalysts were produced and embedded in the polysaccharide matrix of chitosan/curdlan/hydroxyapatite biomaterial. Then, basic physicochemical and structural characterization of the NPs and biomaterials was performed. The primary aim of this work was to evaluate the impact of the combined action of cold nitrogen plasma and the materials produced on proliferation and osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells (ADSCs), which were seeded onto the bone scaffolds containing NPs or Fe(x)O(y)/NPs catalysts. Incorporation of catalysts into the structure of the biomaterial was expected to enhance the formation of plasma-induced RONS, thereby improving stem cell behavior. The results obtained clearly demonstrated that short-time (16s) exposure of ADSCs to nitrogen plasma accelerated proliferation of cells grown on the biomaterial containing Fe(x)O(y)/NPs catalysts and increased osteocalcin production by the cells cultured on the scaffold containing pure NPs. Plasma activation of Fe(x)O(y)/NPs-loaded biomaterial resulted in the formation of appropriate amounts of oxygen-based reactive species that had positive impact on stem cell proliferation and at the same time did not negatively affect their osteogenic differentiation. Therefore, plasma-activated Fe(x)O(y)/NPs-loaded biomaterial is characterized by improved biocompatibility and has great clinical potential to be used in regenerative medicine applications to improve bone healing process.
Filiaciones:
Przekora A:
Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1 Street, 20-093 Lublin, Poland
Audemar M:
IMCN Institute, Université Catholique de Louvain, Place Louis Pasteur 1, 1348 Louvain-la-Neuve, Belgium
Pawlat J:
Chair of Electrical Engineering and Electrotechnologies, Lublin University of Technology, Nadbystrzycka 38a, 20-618 Lublin, Poland
Canal-Barnils C:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Universitat Politècnica de Catalunya (UPC), Av. Eduard Maristany 14, 08930 Barcelona, Spain
Barcelona Research Center in Multiscale Science and Engineering, UPC, 08019 Barcelona, Spain
Research Centre for Biomedical Engineering (CREB), UPC, 08019 Barcelona, Spain
Thomann JS:
Material Research and Technology (MRT) Department, Luxembourg Institute of Science and Technology (LIST), 41 rue du Brill, L-4422 Belvaux, Luxembourg
Labay C:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Universitat Politècnica de Catalunya (UPC), Av. Eduard Maristany 14, 08930 Barcelona, Spain
Barcelona Research Center in Multiscale Science and Engineering, UPC, 08019 Barcelona, Spain
Research Centre for Biomedical Engineering (CREB), UPC, 08019 Barcelona, Spain
Wojcik M:
Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1 Street, 20-093 Lublin, Poland
Kwiatkowski M:
Chair of Electrical Engineering and Electrotechnologies, Lublin University of Technology, Nadbystrzycka 38a, 20-618 Lublin, Poland
Terebun P:
Chair of Electrical Engineering and Electrotechnologies, Lublin University of Technology, Nadbystrzycka 38a, 20-618 Lublin, Poland
Ginalska G:
Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1 Street, 20-093 Lublin, Poland
Hermans S:
IMCN Institute, Université Catholique de Louvain, Place Louis Pasteur 1, 1348 Louvain-la-Neuve, Belgium
Duday D:
Material Research and Technology (MRT) Department, Luxembourg Institute of Science and Technology (LIST), 41 rue du Brill, L-4422 Belvaux, Luxembourg
Open Access
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