Engineering DNA-Grafted Quatsomes as Stable Nucleic Acid-Responsive Fluorescent Nanovesicles
Por:
Rossetti, M, Stella, L, Morla-Folch, J, Bobone, S, Boloix, A, Baranda, L, Moscone, D, Roldan-Molina M, Veciana, J, Segura, MF, Kober, M, Ventosa, N and Porchetta, A
Publicada:
1 nov 2021
Ahead of Print:
1 ago 2021
Resumen:
The development of artificial vesicles into responsive architectures capable of sensing the biological environment and simultaneously signaling the presence of a specific target molecule is a key challenge in a range of biomedical applications from drug delivery to diagnostic tools. Herein, the rational design of biomimetic DNA-grafted quatsome (QS) nanovesicles capable of translating the binding of a target molecule to amphiphilic DNA probes into an optical output is presented. QSs are synthetic lipid-based nanovesicles able to confine multiple organic dyes at the nanoscale, resulting in ultra-bright soft materials with attractiveness for sensing applications. Dye-loaded QS nanovesicles of different composition and surface charge are grafted with fluorescent amphiphilic nucleic acid-based probes to produce programmable FRET-active nanovesicles that operate as highly sensitive signal transducers. The photophysical properties of the DNA-grafted nanovesicles are characterized and the highly selective, ratiometric detection of clinically relevant microRNAs with sensitivity in the low nanomolar range are demonstrated. The potential applications of responsive QS nanovesicles for biosensing applications but also as functional nanodevices for targeted biomedical applications is envisaged.
Filiaciones:
Rossetti, M:
Univ Roma Tor Vergata, Dept Chem Sci & Technol, Via Ric Sci, I-00133 Rome, Italy
Stella, L:
Univ Roma Tor Vergata, Dept Chem Sci & Technol, Via Ric Sci, I-00133 Rome, Italy
Morla-Folch, J:
Inst Ciencia Mat Barcelona, ICMAB, CSIC, Campus UAB, Bellaterra 08193, Spain
Bobone, S:
Univ Roma Tor Vergata, Dept Chem Sci & Technol, Via Ric Sci, I-00133 Rome, Italy
Boloix, A:
UAB, Lab Translat Res Childhood & Adolescent Canc, Vall Hebron Res Inst VHIR, Barcelona, Spain
Baranda, L:
Univ Roma Tor Vergata, Dept Chem Sci & Technol, Via Ric Sci, I-00133 Rome, Italy
Moscone, D:
Univ Roma Tor Vergata, Dept Chem Sci & Technol, Via Ric Sci, I-00133 Rome, Italy
Roldan-Molina M:
Hosp St Joan, Unitat Microscdpia Confocal & Imatge Cellular, Serv Med Genet & Mol, Inst Pediat Malalties Rares IPER, Barcelona 08950, Spain
Inst Recerca St Joan Deu, Barcelona, Spain
Veciana, J:
Inst Ciencia Mat Barcelona, ICMAB, CSIC, Campus UAB, Bellaterra 08193, Spain
Ctr Invest Biomed Red CIBER BBN, Madrid 28029, Spain
Nanomol Technol SL, MOdul Recerca B,Campus Univ Bellaterra, Cerdanyola Del Valles 08950, Spain
Segura, MF:
UAB, Lab Translat Res Childhood & Adolescent Canc, Vall Hebron Res Inst VHIR, Barcelona, Spain
Kober, M:
Inst Ciencia Mat Barcelona, ICMAB, CSIC, Campus UAB, Bellaterra 08193, Spain
Ctr Invest Biomed Red CIBER BBN, Madrid 28029, Spain
Ventosa, N:
Inst Ciencia Mat Barcelona, ICMAB, CSIC, Campus UAB, Bellaterra 08193, Spain
Ctr Invest Biomed Red CIBER BBN, Madrid 28029, Spain
Nanomol Technol SL, MOdul Recerca B,Campus Univ Bellaterra, Cerdanyola Del Valles 08950, Spain
Porchetta, A:
Univ Roma Tor Vergata, Dept Chem Sci & Technol, Via Ric Sci, I-00133 Rome, Italy
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