Dynamics of Reverse Transcription-Polymerase Chain Reaction and Serologic Test Results in Children with SARS-CoV-2 Infection
Por:
Tagarro A, Sanz-Santaeufemia FJ, Grasa C, Cobos E, Yebra J, Alonso-Cadenas JA, Baquero-Artigao F, Mesa GJ, Perez SB, Calvo C, Herreros ML, Epalza C, Melendo S, Dominguez-Rodriguez S, Vidal P, Pacheco M, Ballesteros A, Bernardino M, Villanueva-Medina S, Rodríguez MP, Miragaya CS, Rivière J, Garcés R, Santiago B, Fumadó V, Urretavizcaya-Martínez M, García-García ML, Penín M, Cava F, Sáez E, Iglesias-Bouzas MI, Herrero B, Reinoso T and Moraleda C
Publicada:
1 feb 2022
Ahead of Print:
1 dic 2021
Categoría:
Pediatrics, perinatology and child health
Resumen:
Objectives To determine the time to reverse transcription-polymerase chain reaction (RT-PCR) negativity after the first positive RT-PCR test, factors associated with longer time to RT-PCR negativity, proportion of children seroconverting after proven severe acute respiratory syndrome coronavirus 2 infection, and factors associated with the lack of seroconversion.
Study design The Epidemiological Study of Coronavirus in Children of the Spanish Society of Pediatrics is a multicenter study conducted in Spanish children to assess the characteristics of coronavirus disease 2019. In a subset of patients, 3 serial RT-PCR tests on nasopharyngeal swab specimens were performed after the first RTPCR test, and immunoglobulin G serology for severe acute respiratory syndrome coronavirus 2 antibodies was performed in the acute and follow-up (<14 and >= 14 days after diagnosis) phase.
Results In total, 324 patients were included in the study. The median time to RT-PCR negativity was 17 days (IQR, 8-29 days), and 35% of patients remained positive more than 4 weeks after the first RT-PCR test. The probability of RT-PCR negativity did not differ across groups defined by sex, disease severity, immunosuppressive drugs, or clinical phenotype. Globally, 24% of children failed to seroconvert after infection. Seroconversion was associated with hospitalization, persistence of RT-PCR positivity, and days of fever.
Conclusions Time to RT-PCR negativity was long, regardless of the severity of symptoms or other patient features. This finding should be considered when interpreting RT-PCR results in a child with symptoms, especially those with mild symptoms. Seroprevalence and postimmunization studies should consider that 11 in 4 infected children fail to seroconvert.
Filiaciones:
Tagarro A:
Pediatrics Department. Hospital Universitario Infanta Sofía Madrid, Spain
Pediatrics Research Group. Universidad Europea de Madrid, Spain
Fundación de Investigación Biomédica Hospital 12 de Octubre. Instituto de Investigación 12 de Octubre (imas12), Madrid, Spain. RITIP (Translational Research Network in Paediatric Infectious Diseases)
Sanz-Santaeufemia FJ:
Pediatrics Department, Hospital Infantil Universitario Niño Jesús, Madrid, Spain
Grasa C:
Pediatrics, Infectious and Tropical Diseases Department. Hospital Universitario La Paz. Instituto Investigación Hospital La Paz (IDIPaz), Madrid, Spain. RITIP (Translational Research Network in Paediatric Infectious Diseases)
Cobos E:
Fundación de Investigación Biomédica Hospital 12 de Octubre. Instituto de Investigación 12 de Octubre (imas12), Madrid, Spain. RITIP (Translational Research Network in Paediatric Infectious Diseases)
Yebra J:
Pediatrics Department. Hospital Universitario Infanta Sofía Madrid, Spain
Alonso-Cadenas JA:
Emergency Department, Hospital Infantil Universitario Niño Jesús, Madrid, Spain
Baquero-Artigao F:
Pediatrics, Infectious and Tropical Diseases Department. Hospital Universitario La Paz. Instituto Investigación Hospital La Paz (IDIPaz), Madrid, Spain. RITIP (Translational Research Network in Paediatric Infectious Diseases)
Mesa GJ:
Pediatrics Department. Hospital Universitario Infanta Sofía Madrid, Spain
Perez SB:
Pediatrics Department. Hospital Universitario Infanta Sofía Madrid, Spain
Calvo C:
Pediatrics, Infectious and Tropical Diseases Department. Hospital Universitario La Paz. Instituto Investigación Hospital La Paz (IDIPaz), Madrid, Spain. RITIP (Translational Research Network in Paediatric Infectious Diseases)
Herreros ML:
Pediatrics Department. Hospital Universitario Infanta Sofía Madrid, Spain
Epalza C:
Fundación de Investigación Biomédica Hospital 12 de Octubre. Instituto de Investigación 12 de Octubre (imas12), Madrid, Spain. RITIP (Translational Research Network in Paediatric Infectious Diseases)
Pediatrics Department, Hospital Universitario 12 de Octubre, Madrid, Spain
Melendo S:
Infectious Diseases and Pediatric Immunology Unit. Department of Pediatrics, Hospital Universitario Vall d'Hebron. Barcelona, Spain
Dominguez-Rodriguez S:
Fundación de Investigación Biomédica Hospital 12 de Octubre. Instituto de Investigación 12 de Octubre (imas12), Madrid, Spain. RITIP (Translational Research Network in Paediatric Infectious Diseases)
Vidal P:
Pediatrics Department. Hospital Clínico Universitario Lozano Blesa Zaragoza, Spain
Pacheco M:
Pediatrics Department. Hospital Universitario Infanta Sofía Madrid, Spain
Ballesteros A:
Fundación de Investigación Biomédica Hospital 12 de Octubre. Instituto de Investigación 12 de Octubre (imas12), Madrid, Spain. RITIP (Translational Research Network in Paediatric Infectious Diseases)
Bernardino M:
Pediatrics Research Group. Universidad Europea de Madrid, Spain
Villanueva-Medina S:
Pediatrics Department, Hospital Universitario 12 de Octubre, Madrid, Spain
Rodríguez MP:
Pediatrics, Infectious and Tropical Diseases Department. Hospital Universitario La Paz. Instituto Investigación Hospital La Paz (IDIPaz), Madrid, Spain. RITIP (Translational Research Network in Paediatric Infectious Diseases)
Miragaya CS:
Emergency Department, Hospital Infantil Universitario Niño Jesús, Madrid, Spain
Rivière J:
Infectious Diseases and Pediatric Immunology Unit. Department of Pediatrics, Hospital Universitario Vall d'Hebron. Barcelona, Spain
Garcés R:
Pediatrics Department. Hospital Clínico Universitario Lozano Blesa Zaragoza, Spain
Santiago B:
Pediatric Infectious Diseases Unit. Department of Pediatrics, Hospital Universitario Gregorio Marañón, Madrid, Spain
Fumadó V:
Pediatric Infectious Diseases Unit. Department of Pediatrics. Hospital Universitario Sant Joan de Deu Barcelona, Spain
Urretavizcaya-Martínez M:
Pediatrics Department, Complejo Hospitalario de Navarra, Pamplona, Spain
García-García ML:
Pediatrics Department, Hospital Universitario Severo Ochoa Madrid, Madrid, Spain
Penín M:
Pediatrics Departament. Hospital Universitario Príncipe de Asturias, Madrid, Spain
Cava F:
Microbiology Department, UR Salud, Hospital Universitario Infanta Sofía, Madrid, Spain
Sáez E:
Microbiology Department, UR Salud, Hospital Universitario Infanta Sofía, Madrid, Spain
Iglesias-Bouzas MI:
Pediatric Intensive Care Unit, Hospital Infantil Universitario Niño Jesús, Madrid, Spain
Herrero B:
Oncology Department, Hospital Infantil Universitario Niño Jesús, Madrid, Spain
Reinoso T:
Pediatrics Department. Hospital Universitario Infanta Sofía Madrid, Spain
Moraleda C:
Fundación de Investigación Biomédica Hospital 12 de Octubre. Instituto de Investigación 12 de Octubre (imas12), Madrid, Spain. RITIP (Translational Research Network in Paediatric Infectious Diseases)
Pediatrics Department, Hospital Universitario 12 de Octubre, Madrid, Spain
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