Development of topical eye-drops of lactoferrin-loaded biodegradable nanoparticles for the treatment of anterior segment inflammatory processes
Por:
López-Machado A, Díaz N, Cano, Amanda, Espina M, Badia-Palacin J, Baldoma L, Cristina Calpena A, Biancardi M, Souto EB, Luisa García M and Sánchez-López E
Publicada:
20 nov 2021
Ahead of Print:
1 oct 2021
Resumen:
Ocular inflammation is one of the most common comorbidities associated to ophthalmic surgeries and disorders. Since conventional topical ophthalmic treatments present disadvantages such as low bioavailability and relevant side effects, natural alternatives constitute an unmet medical need. In this sense, lactoferrin, a high molecular weight protein, is a promising alternative against inflammation. However, lactoferrin aqueous instability and high nasolacrimal duct drainage compromises its potential effectiveness. Moreover, nanotechnology has led to an improvement in the administration of active compounds with compromised biopharmaceutical profiles. Here, we incorporate lactoferrin into biodegradable polymeric nanoparticles and optimized the formulation using the design of experiments approach. A monodisperse nanoparticles population was obtained with an average size around 130 nm and positive surface charge. Pharmacokinetic and pharmacodynamic behaviour were improved by the nanoparticles showing a prolonged lactoferrin release profile. Lactoferrin nanoparticles were noncytotoxic and non-irritant neither in vitro nor in vivo. Moreover, nanoparticles exhibited significantly increased anti-inflammatory efficacy in cell culture and preclinical assays. In conclusion, lactoferrin loaded nanoparticles constitute a safe and novel nanotechnological tool suitable for the treatment of ocular inflammation.
Filiaciones:
López-Machado A:
Department of Pharmacy, Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, Barcelona, Spain
Institute of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, Barcelona, Spain
Díaz N:
Department of Biochemistry & Physiology, Faculty of Pharmacy & Food Sciences, University of Barcelona, Barcelona, Spain
Institute of Biomedicine, University of Barcelona (IBUB), Barcelona, Spain
Institut de Recerca Sant Joan de Deu (IRSJD), Barcelona, Spain
Cano, Amanda:
Department of Pharmacy, Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, Barcelona, Spain
Institute of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, Barcelona, Spain
Biomedical Research Networking Centre in Neurodegenerative Diseases (CIBERNED), Madrid, Spain
Espina M:
Department of Pharmacy, Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, Barcelona, Spain
Institute of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, Barcelona, Spain
Badia-Palacin J:
Department of Biochemistry & Physiology, Faculty of Pharmacy & Food Sciences, University of Barcelona, Barcelona, Spain
Institute of Biomedicine, University of Barcelona (IBUB), Barcelona, Spain
Institut de Recerca Sant Joan de Deu (IRSJD), Barcelona, Spain
Baldoma L:
Department of Biochemistry & Physiology, Faculty of Pharmacy & Food Sciences, University of Barcelona, Barcelona, Spain
Institute of Biomedicine, University of Barcelona (IBUB), Barcelona, Spain
Institut de Recerca Sant Joan de Deu (IRSJD), Barcelona, Spain
Cristina Calpena A:
Department of Pharmacy, Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, Barcelona, Spain
Institute of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, Barcelona, Spain
Biancardi M:
Technology Dedicated to Care, Barcelona, Spain
Souto EB:
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, Portugal
CEB-Centre of Biological Engineering, Campus de Gualtar, University of Minho, 4710-057 Braga, Portugal
Luisa García M:
Department of Pharmacy, Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, Barcelona, Spain
Institute of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, Barcelona, Spain
Biomedical Research Networking Centre in Neurodegenerative Diseases (CIBERNED), Madrid, Spain
Sánchez-López E:
Department of Pharmacy, Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, Barcelona, Spain
Institute of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, Barcelona, Spain
Institute of Biomedicine, University of Barcelona (IBUB), Barcelona, Spain
Green Published, hybrid
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