Effects of Hypothermia and Allopurinol on Oxidative Status in a Rat Model of Hypoxic Ischemic Encephalopathy
Por:
Durán Fernández-Feijóo C, Rodríguez-Fanjul J, López-Abad M, Hadley S, Cavia-Saiz M, Muñiz P, Arnaez J, Fernández-Lorenzo JR and Camprubí-Camprubí M
Publicada:
1 oct 2021
Ahead of Print:
25 sep 2021
Resumen:
Hypoxic ischemic encephalopathy (HIE) is one of the main causes of morbidity and mortality during the neonatal period, despite treatment with hypothermia. There is evidence that oxidative damage plays an important role in the pathophysiology of hypoxic-ischemic (HI) brain injury. Our aim was to investigate whether postnatal allopurinol administration in combination with hypothermia would reduce oxidative stress (OS) biomarkers in an animal model of HIE. Postnatal 10-day rat pups underwent unilateral HI of moderate severity. Pups were randomized into: Sham operated, hypoxic-ischemic (HI), HI + allopurinol (HIA), HI + hypothermia (HIH), and HI + hypothermia + allopurinol (HIHA). Biomarkers of OS and antioxidants were evaluated: GSH/GSSG ratio and carbonyl groups were tested in plasma. Total antioxidant capacity (TAC) was analyzed in plasma and cerebrospinal fluid, and 8-iso-prostaglandin F2 alpha was measured in brain tissue. Plasma 2,2 '-azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) levels were preserved in those groups that received allopurinol and dual therapy. In cerebrospinal fluid, only the HIA group presented normal ferric reducing ability of plasma (FRAP) levels. Protein oxidation and lipid peroxidation were significantly reduced in all groups treated with hypothermia and allopurinol, thus enhancing neuroprotection in HIE.
Filiaciones:
Durán Fernández-Feijóo C:
Department of Neonatology, Hospital Álvaro Cunqueiro, EOXI, 36312 Vigo, Spain
Rodríguez-Fanjul J:
Neonatal Intensive Care Unit, Paediatrics Department, Hospital Germans Trias i Pujol, Universitat Autonoma de Barcelona, 08916 Badalona, Spain
López-Abad M:
Department of Neonatology, BCNatal|Barcelona Center for Maternal Fetal and Neonatal Medicine Hospital Sant Joan de Déu and Hospital Clínic, University of Barcelona, 08950 Esplugues de Llobregat, Spain
Hadley S:
Vanderbilt University School of Medicine, Nashville, TN 37232, USA
Cavia-Saiz M:
Department of Biotechnology and Food Science, Facutlty of Sciences, University of Burgos, 09001 Burgos, Spain
Muñiz P:
Department of Biotechnology and Food Science, Facutlty of Sciences, University of Burgos, 09001 Burgos, Spain
Arnaez J:
Department of Neonatology, Hospital Universitario de Burgos, NeNe Foundation, 09006 Burgos, Spain
Fernández-Lorenzo JR:
Department of Neonatology, Hospital Álvaro Cunqueiro, EOXI, 36312 Vigo, Spain
Camprubí-Camprubí M:
Department of Neonatology, BCNatal|Barcelona Center for Maternal Fetal and Neonatal Medicine Hospital Sant Joan de Déu and Hospital Clínic, University of Barcelona, 08950 Esplugues de Llobregat, Spain
Green Submitted, gold
|