Mimicking Bone Extracellular Matrix: From BMP-2-Derived Sequences to Osteogenic-Multifunctional Coatings
Por:
Oliver-Cervelló Ll, Martin-Gómez H, Mandakhbayar N, Jo YW, Cavalcanti-Adam EA, Kim HW, Ginebra MP, Lee JH and Mas C
Publicada:
1 oct 2022
Ahead of Print:
1 ago 2022
Resumen:
Cell-material interactions are regulated by mimicking bone extracellular matrix on the surface of biomaterials. In this regard, reproducing the extracellular conditions that promote integrin and growth factor (GF) signaling is a major goal to trigger bone regeneration. Thus, the use of synthetic osteogenic domains derived from bone morphogenetic protein 2 (BMP-2) is gaining increasing attention, as this strategy is devoid of the clinical risks associated with this molecule. In this work, the wrist and knuckle epitopes of BMP-2 are screened to identify peptides with potential osteogenic properties. The most active sequences (the DWIVA motif and its cyclic version) are combined with the cell adhesive RGD peptide (linear and cyclic variants), to produce tailor-made biomimetic peptides presenting the bioactive cues in a chemically and geometrically defined manner. Such multifunctional peptides are next used to functionalize titanium surfaces. Biological characterization with mesenchymal stem cells demonstrates the ability of the biointerfaces to synergistically enhance cell adhesion and osteogenic differentiation. Furthermore, in vivo studies in rat calvarial defects prove the capacity of the biomimetic coatings to improve new bone formation and reduce fibrous tissue thickness. These results highlight the potential of mimicking integrin-GF signaling with synthetic peptides, without the need for exogenous GFs.
Filiaciones:
Oliver-Cervelló Ll:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Universitat Politècnica de Catalunya (UPC), Barcelona, 08019, Spain
Barcelona Research Center in Multiscale Science and Engineering, UPC, Barcelona, 08019, Spain
Martin-Gómez H:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Universitat Politècnica de Catalunya (UPC), Barcelona, 08019, Spain
Barcelona Research Center in Multiscale Science and Engineering, UPC, Barcelona, 08019, Spain
Mandakhbayar N:
Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, 330-714, Republic of Korea
Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, 330-714, Republic of Korea
Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan, 330-714, Republic of Korea
Jo YW:
Neobiotech Co., Ltd R&D Center, Seoul, 08381, Republic of Korea
Cavalcanti-Adam EA:
Department of Cellular Biophysics, Growth Factor Mechanobiology group, Max Planck Institute for Medical Research Jahnstraße 29, 69120, Heidelberg, Germany
Kim HW:
Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, 330-714, Republic of Korea
Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, 330-714, Republic of Korea
Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan, 330-714, Republic of Korea
Ginebra MP:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Universitat Politècnica de Catalunya (UPC), Barcelona, 08019, Spain
Barcelona Research Center in Multiscale Science and Engineering, UPC, Barcelona, 08019, Spain
Institute for Bioengineering of Catalonia, Barcelona, 08028, Spain
Lee JH:
Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, 330-714, Republic of Korea
Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, 330-714, Republic of Korea
Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan, 330-714, Republic of Korea
Mas C:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Universitat Politècnica de Catalunya (UPC), Barcelona, 08019, Spain
Barcelona Research Center in Multiscale Science and Engineering, UPC, Barcelona, 08019, Spain
hybrid
|