Plasmodium falciparum sexual conversion rates can be affected by artemisinin-based treatment in naturally infected malaria patients
Por:
Portugaliza HP, Natama HM, Guetens P, Rovira-Vallbona E, Somé AM, Millogo A, Ouédraogo DF, Valéa I, Sorgho H, Tinto H, van Hong N, Sitoe A, Varo R, Bassat Q, Cortés A and Rosanas-Urgell A
Publicada:
1 sep 2022
Ahead of Print:
9 ago 2022
Resumen:
Background Artemisinins (ART) are the key component of the frontline antimalarial treatment, but their impact on Plasmodium falciparum sexual conversion rates in natural malaria infections remains unknown. This is an important knowledge gap because sexual conversion rates determine the relative parasite investment between maintaining infection in the same human host and transmission to mosquitoes.Methods The primary outcome of this study was to assess the impact of ART-based treatment on sexual conversion rates by comparing the relative transcript levels of pfap2-g and other sexual ring biomarkers (SRBs) before and after treatment. We analysed samples from previously existing cohorts in Vietnam, Burkina Faso and Mozambique (in total, n=109) collected before treatment and at 12 h intervals after treatment. As a secondary objective, we investi-gated factors that may influence the effect of treatment on sexual conversion rates.Findings In the majority of infections from the African cohorts, but not from Vietnam, we observed increased expression of pfap2-g and other SRBs after treatment. Estimated parasite age at the time of treatment was negatively correlated with the increase in pfap2-g transcript levels, suggesting that younger parasites are less susceptible to stim-ulation of sexual conversion.Interpretation We observed enhanced expression of SRBs after ART-based treatment in many patients, which sug-gests that in natural malaria infections sexual conversion rates can be altered by treatment. ART-based treatment reduces the potential of a treated individual to transmit the disease, but we hypothesise that under some circumstan-ces this reduction may be attenuated by ART-enhanced sexual conversion.Funding Spanish Agencia Estatal de Investigaci?on (AEI), European Regional Development Fund (ERDF, European Union), Belgium Development Cooperation (DGD), Canadian University Health Network (UHN), TransGlobal-Health-Erasmus Mundus (European Union).Copyright (c) 2022 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/) eBioMedicine 104198 Published online https://doi.org/10.1016/j. ebiom.2022.104198
Filiaciones:
Portugaliza HP:
ISGlobal, Hospital Clinic - Universitat de Barcelona, Barcelona, Catalonia, Spain
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium
Department of Global Health, Amsterdam University Medical Centers, location Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
Natama HM:
Unité de Recherche Clinique de Nanoro, Institut de Recherche en Sciences de la Santé, Nanoro, Burkina Faso
Guetens P:
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium
Rovira-Vallbona E:
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium
Somé AM:
Unité de Recherche Clinique de Nanoro, Institut de Recherche en Sciences de la Santé, Nanoro, Burkina Faso
Millogo A:
Unité de Recherche Clinique de Nanoro, Institut de Recherche en Sciences de la Santé, Nanoro, Burkina Faso
Ouédraogo DF:
Unité de Recherche Clinique de Nanoro, Institut de Recherche en Sciences de la Santé, Nanoro, Burkina Faso
Valéa I:
Unité de Recherche Clinique de Nanoro, Institut de Recherche en Sciences de la Santé, Nanoro, Burkina Faso
Sorgho H:
Unité de Recherche Clinique de Nanoro, Institut de Recherche en Sciences de la Santé, Nanoro, Burkina Faso
Tinto H:
Unité de Recherche Clinique de Nanoro, Institut de Recherche en Sciences de la Santé, Nanoro, Burkina Faso
van Hong N:
National Institute of Malariology, Parasitology and Entomology (NIMPE), Hanoi, Vietnam
Sitoe A:
Centro de Investigação em Saúde de Manhiça (CISM), Maputo, Mozambique
:
ISGlobal, Hospital Clinic - Universitat de Barcelona, Barcelona, Catalonia, Spain
Centro de Investigação em Saúde de Manhiça (CISM), Maputo, Mozambique
Bassat Q:
ISGlobal, Hospital Clinic - Universitat de Barcelona, Barcelona, Catalonia, Spain
Centro de Investigação em Saúde de Manhiça (CISM), Maputo, Mozambique
ICREA, Barcelona, Catalonia, Spain
Pediatrics Department, Hospital Sant Joan de Déu, Universitat de Barcelona, Esplugues, Barcelona, Catalonia, Spain
Consorcio de Investigación Biomédica en Red de Epidemiología y Salud Pública (CIBERESP), Madrid, Spain
Cortés A:
ISGlobal, Hospital Clinic - Universitat de Barcelona, Barcelona, Catalonia, Spain
ICREA, Barcelona, Catalonia, Spain
Rosanas-Urgell A:
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium
ISGlobal, Hospital Clinic - Universitat de Barcelona, Barcelona, Catalonia, Spain; Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium; Department of Global Health, Amsterdam University Medical Centers, location Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.; Unite de Recherche Clinique de Nanoro, Institut de Recherche en Sciences de la Sante, Nanoro, Burkina Faso.; Department of Biomedical Sciences, Institute of Tropical Medicine, A
Green Submitted, gold
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