TSPO PET Imaging as a Potent Non-Invasive Biomarker for Diffuse Intrinsic Pontine Glioma in a Patient-Derived Orthotopic Rat Model
Por:
Chevaleyre, C, Kereselidze, D, Caille, F, Tournier, N, Gene-Olaciregui N, Winkeler, A, Decleves, X, Jego, B, Cisternino, S, Auvity, S and Truillet, C
Publicada:
1 oct 2022
Ahead of Print:
18 oct 2022
Resumen:
Diffuse intrinsic pontine gliomas (DIPG), the first cause of cerebral pediatric cancer death, will greatly benefit from specific and non-invasive biomarkers for patient follow-up and monitoring of drug efficacy. Since biopsies are challenging for brain tumors, molecular imaging may be a technique of choice to target and follow tumor evolution. So far, MR remains the imaging technique of reference for DIPG, although it often fails to define the extent of tumors, an essential parameter for therapeutic efficacy assessment. Thanks to its high sensitivity, positron emission tomography (PET) offers a unique way to target specific biomarkers in vivo. We demonstrated in a patient-derived orthotopic xenograft (PDOX) model in the rat that the translocator protein of 18 kDa (TSPO) may be a promising biomarker for monitoring DIPG tumors. We studied the distribution of 18F-DPA-714, a TSPO radioligand, in rats inoculated with HSJD-DIPG-007 cells. The primary DIPG human cell line HSJD-DIPG-007 highly represents this pediatric tumor, displaying the most prevalent DIPG mutations, H3F3A (K27M) and ACVR1 (R206H). Kinetic modeling and parametric imaging using the brain 18F-DPA-714 PET data enabled specific delineation of the DIPG tumor area, which is crucial for radiotherapy dose management.
Filiaciones:
Chevaleyre, C:
Univ Paris Saclay, INSERM, CNRS, BioMaps,Serv Hosp Frederic Joliot,CEA, 4 Pl Gen Leclerc, F-91401 Orsay, France
Kereselidze, D:
Univ Paris Saclay, INSERM, CNRS, BioMaps,Serv Hosp Frederic Joliot,CEA, 4 Pl Gen Leclerc, F-91401 Orsay, France
Caille, F:
Univ Paris Saclay, INSERM, CNRS, BioMaps,Serv Hosp Frederic Joliot,CEA, 4 Pl Gen Leclerc, F-91401 Orsay, France
Tournier, N:
Univ Paris Saclay, INSERM, CNRS, BioMaps,Serv Hosp Frederic Joliot,CEA, 4 Pl Gen Leclerc, F-91401 Orsay, France
Gene-Olaciregui N:
St Joan de Deu Hosp, Inst Recerca, Carrer St Quinti 77, Barcelona 08041, Spain
Winkeler, A:
Univ Paris Saclay, INSERM, CNRS, BioMaps,Serv Hosp Frederic Joliot,CEA, 4 Pl Gen Leclerc, F-91401 Orsay, France
Decleves, X:
Univ Paris Cite, Inserm UMR S1144, F-75006 Paris, France
Jego, B:
Univ Paris Saclay, INSERM, CNRS, BioMaps,Serv Hosp Frederic Joliot,CEA, 4 Pl Gen Leclerc, F-91401 Orsay, France
Cisternino, S:
Univ Paris Cite, Inserm UMR S1144, F-75006 Paris, France
Hop Univ Necker Enfants Malad, AP HP, 149 Rue Sevres, F-75015 Paris, France
Auvity, S:
Univ Paris Saclay, INSERM, CNRS, BioMaps,Serv Hosp Frederic Joliot,CEA, 4 Pl Gen Leclerc, F-91401 Orsay, France
Univ Paris Cite, Inserm UMR S1144, F-75006 Paris, France
Hop Univ Necker Enfants Malad, AP HP, 149 Rue Sevres, F-75015 Paris, France
Truillet, C:
Univ Paris Saclay, INSERM, CNRS, BioMaps,Serv Hosp Frederic Joliot,CEA, 4 Pl Gen Leclerc, F-91401 Orsay, France
gold, Green Published
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