Surface competition between osteoblasts and bacteria on silver-doped bioactive titanium implant
Por:
Piñera-Avellaneda D, Buxadera J, Ginebra MP, Calero JA, Manero JM and Rupérez E
Publicada:
1 mar 2023
Ahead of Print:
1 ene 2023
Resumen:
The rapid integration in the bone tissue and the prevention of bacterial infection are key for the success of the implant. In this regard, a silver (Ag)-doped thermochemical treatment that generate an Ag-doped calcium titanate layer on titanium (Ti) implants was previously developed by our group to improve the bone-bonding ability and provide antibacterial activity. In the present study, the biological and antibacterial potential of this coating has been further studied. In order to prove that the Ag-doped layer has an antibacterial effect with no detrimental effect on the bone cells, the behavior of osteoblast-like cells in terms of cell adhesion, morphology, proliferation and differentiation was evaluated, and the biofilm inhibition capacity was assessed. Moreover, the competition by the surface between cell and bacteria was carried out in two different co-culture methods. Finally, the treatment was applied to porous Ti implants to study in vivo osteointegration. The results show that the incorporation of Ag inhibits the biofilm formation and has no effect on the performance of osteoblast-like cells. Therefore, it can be concluded that the Ag-doped surface is capable of preventing bone bacterial infection and providing suitable osseointegration.
Filiaciones:
Piñera-Avellaneda D:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Technical University of Catalonia (UPC), Barcelona East School of Engineering (EEBE), 08019 Barcelona, Spain
Barcelona Research Center in Multiscale Science and Engineering, UPC, EEBE, 08019 Barcelona, Spain
Institut de Recerca Sant Joan de Déu, 08034 Barcelona, Spain
Buxadera J:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Technical University of Catalonia (UPC), Barcelona East School of Engineering (EEBE), 08019 Barcelona, Spain
Barcelona Research Center in Multiscale Science and Engineering, UPC, EEBE, 08019 Barcelona, Spain
Institut de Recerca Sant Joan de Déu, 08034 Barcelona, Spain
Ginebra MP:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Technical University of Catalonia (UPC), Barcelona East School of Engineering (EEBE), 08019 Barcelona, Spain
Barcelona Research Center in Multiscale Science and Engineering, UPC, EEBE, 08019 Barcelona, Spain
Institut de Recerca Sant Joan de Déu, 08034 Barcelona, Spain
Institute for Bioengineering of Catalonia (IBEC), 08028 Barcelona, Spain
Calero JA:
AMES GROUP, 08980 Sant Feliu de Llobregat, Barcelona, Spain
Manero JM:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Technical University of Catalonia (UPC), Barcelona East School of Engineering (EEBE), 08019 Barcelona, Spain
Barcelona Research Center in Multiscale Science and Engineering, UPC, EEBE, 08019 Barcelona, Spain
Institut de Recerca Sant Joan de Déu, 08034 Barcelona, Spain
Rupérez E:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Technical University of Catalonia (UPC), Barcelona East School of Engineering (EEBE), 08019 Barcelona, Spain
Barcelona Research Center in Multiscale Science and Engineering, UPC, EEBE, 08019 Barcelona, Spain
Institut de Recerca Sant Joan de Déu, 08034 Barcelona, Spain
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