Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing
Por:
Blasi D, Gonzalez-Pato N, Rodriguez Rodriguez X, Diez-Zabala I, Srinivasan SY, Camarero N, Esquivias O, Roldan-Molina M, Guasch J, Laromaine A, Gorostiza P, Veciana J and Ratera I
Publicada:
9 ago 2023
Ahead of Print:
1 abr 2023
Resumen:
Ratiometric fluorescent nanothermometers with near-infrared emission play an important role in in vivo sensing since they can be used as intracellular thermal sensing probes with high spatial resolution and high sensitivity, to investigate cellular functions of interest in diagnosis and therapy, where current approaches are not effective. Herein, the temperature-dependent fluorescence of organic nanoparticles is designed, synthesized, and studied based on the dual emission, generated by monomer and excimer species, of the tris(2,4,6-trichlorophenyl)methyl radical (TTM) doping organic nanoparticles (TTMd-ONPs), made of optically neutral tris(2,4,6-trichlorophenyl)methane (TTM-aH), acting as a matrix. The excimer emission intensity of TTMd-ONPs decreases with increasing temperatures whereas the monomer emission is almost independent and can be used as an internal reference. TTMd-ONPs show a great temperature sensitivity (3.4% K-1 at 328 K) and a wide temperature response at ambient conditions with excellent reversibility and high colloidal stability. In addition, TTMd-ONPs are not cytotoxic and their ratiometric outputs are unaffected by changes in the environment. Individual TTMd-ONPs are able to sense temperature changes at the nano-microscale. In vivo thermometry experiments in Caenorhabditis elegans (C. elegans) worms show that TTMd-ONPs can locally monitor internal body temperature changes with spatio-temporal resolution and high sensitivity, offering multiple applications in the biological nanothermometry field.
Filiaciones:
Blasi D:
Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Bellaterra, 08193, Spain
Dipartimento di Chimica, Università degli Studi di Bari "Aldo Moro", Bari, 70125, Italy
Gonzalez-Pato N:
Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Bellaterra, 08193, Spain
Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Campus UAB, Bellaterra, 08193, Spain
Rodriguez Rodriguez X:
Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Bellaterra, 08193, Spain
Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Campus UAB, Bellaterra, 08193, Spain
Diez-Zabala I:
Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Bellaterra, 08193, Spain
Srinivasan SY:
Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Bellaterra, 08193, Spain
Camarero N:
Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology, Clúster, Baldiri Reixac 10-12, Barcelona, 08028, Spain
Esquivias O:
Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Bellaterra, 08193, Spain
Roldan-Molina M:
Unitat de Microscòpia Confocal i Imatge Cellular, Servei de Medicina Genètica i Molecular, Institut Pediàtric de Malaties Rares (IPER), Hospital Sant Joan de Déu, Esplugues de Llobregat, 08950, Spain
Guasch J:
Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Bellaterra, 08193, Spain
Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Campus UAB, Bellaterra, 08193, Spain
Dynamic Biomimetics for Cancer Immunotherapy, Max Planck Partner Group, ICMAB-CSIC, Campus UAB, Bellaterra, 08193, Spain
Laromaine A:
Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Bellaterra, 08193, Spain
Gorostiza P:
Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Campus UAB, Bellaterra, 08193, Spain
Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology, Clúster, Baldiri Reixac 10-12, Barcelona, 08028, Spain
Catalan Institution for Research and Advanced Studies (ICREA), Barcelona, 08010, Spain
Veciana J:
Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Bellaterra, 08193, Spain
Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Campus UAB, Bellaterra, 08193, Spain
Ratera I:
Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Bellaterra, 08193, Spain
Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Campus UAB, Bellaterra, 08193, Spain
Green Submitted, hybrid
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