Reducing bacterial adhesion to titanium surfaces using low intensity alternating electrical pulses
Por:
Bernaus, M, Guillem J, Bermudez-Castel, A, Calero, JA, Torres, D, Veloso, M and Font-Vizcarra, L
Publicada:
18 jun 2022
Categoría:
Orthopedics and sports medicine
Resumen:
BACKGROUND
Orthopedic implant-related infection remains one of the most serious complications after orthopedic surgery. In recent years, there has been an increased scientific interest to improve prevention and treatment strategies. However, many of these strategies have focused on chemical measures.
AIM
To analyze the effect of alternating current electrical fields on bacterial adherence to titanium surfaces.
METHODS
Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) were exposed to 6.5 V electrical currents at different frequencies: 0.5 Hz, 0.1 Hz, and 0.05 Hz. After exposure, a bacterial count was then performed and compared to the control model. Other variables registered included the presence of electrocoagulation of the medium, electrode oxidation and/or corrosion, and changes in pH of the medium.
RESULTS
The most effective electrical model for reducing S. aureus adhesion was 6.5 V alternating current at 0.05 Hz achieving a 90% adhesion reduction rate. For E. coli, the 0.05 Hz frequency model also showed the most effective results with a 53% adhesion reduction rate, although these were significantly lower than S. aureus. Notable adhesion reduction rates were observed for S. aureus and E.coli in the studied conditions. However, the presence of electrode oxidation makes us presume these conditions are not optimal for in vivo use.
CONCLUSION
Although our findings suggest electrical currents may be useful in preventing bacterial adhesion to metal surfaces, further research using other electrical conditions must be examined to consider their use for in vivo trials.
Filiaciones:
Bernaus, M:
Hosp Univ Mutua Terrassa, Dept Orthoped & Traumatol, Osteoarticular Infect Unit, Placa Dr Robert 5, Terrassa 08221, Spain
Guillem J:
Univ Politecn Cataluna, Dept Mat Sci & Met Engn, Barcelona 08930, Spain
Univ Politecn Cataluna, Barcelona Res Ctr Multiscale Sci & Engn, Barcelona 08930, Spain
Bermudez-Castel, A:
Innovat Minds SL, Terrassa 08224, Spain
Calero, JA:
AMES PM Tech Ctr, St Vicenc Dels Horts 08980, Spain
Torres, D:
AMES PM Tech Ctr, St Vicenc Dels Horts 08980, Spain
Veloso, M:
Hosp Univ Mutua Terrassa, Dept Orthoped & Traumatol, Osteoarticular Infect Unit, Placa Dr Robert 5, Terrassa 08221, Spain
Font-Vizcarra, L:
Hosp Univ Mutua Terrassa, Dept Orthoped & Traumatol, Osteoarticular Infect Unit, Placa Dr Robert 5, Terrassa 08221, Spain
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