Gallium and silver-doped titanium surfaces provide enhanced osteogenesis, reduce bone resorption and prevent bacterial infection in co-culture
Por:
Piñera-Avellaneda D, Buxadera J, Delint RC, Dalby MJ, Burgess KV, Ginebra MP, Rupérez E and Manero JM
Publicada:
1 may 2024
Ahead of Print:
1 may 2024
Resumen:
Bacterial infection remains a significant problem associated with orthopaedic surgeries leading to surgical site infection (SSI). This unmet medical need can become an even greater complication when surgery is due to malignant bone tumor. In the present study, we evaluated in vitro titanium (Ti) implants subjected to gallium (Ga) and silver (Ag)-doped thermochemical treatment as strategy to prevent SSI and improve osteointegration in bone defects caused by diseases such as osteoporosis, bone tumor, or bone metastasis. Firstly, as Ga has been reported to be an osteoinductive and anti-resorptive agent, its performance in the mixture was proved by studying human mesenchymal stem cells (hMSC) and pre-osteoclasts (RAW264.7) behaviour. Then, the antibacterial potential provided by Ag was assessed by resembling "The Race for the Surface" between hMSC and Pseudomonas aeruginosa in two co-culture methods. Moreover, the presence of quorum sensing molecules in the co-culture was evaluated. The results highlighted the suitability of the mixture to induce osteodifferentiation and reduce osteoclastogenesis in vitro . Furthermore, the GaAg surface promoted strong survival rate and retained osteoinduction potential of hMSCs even after bacterial inoculation. Therefore, GaAg-modified titanium may be an ideal candidate to repair bone defects caused by excessive bone resorption, in addition to preventing SSI.
Filiaciones:
Piñera-Avellaneda D:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Technical University of Catalonia (UPC), Barcelona East School of Engineering (EEBE), 08019 Barcelona, Spain
Barcelona Research Center in Multiscale Science and Engineering, UPC, EEBE, 08019, Barcelona, Spain
Institut de Recerca Sant Joan de Déu, 08034, Barcelona, Spain
Buxadera J:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Technical University of Catalonia (UPC), Barcelona East School of Engineering (EEBE), 08019 Barcelona, Spain
Barcelona Research Center in Multiscale Science and Engineering, UPC, EEBE, 08019, Barcelona, Spain
Institut de Recerca Sant Joan de Déu, 08034, Barcelona, Spain
Delint RC:
Centre for the Cellular Microenvironment, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK
Dalby MJ:
Centre for the Cellular Microenvironment, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK
Burgess KV:
EdinOmics, University of Edinburgh, Max Born Crescent, Edinburgh, EH9 3BF, UK
Ginebra MP:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Technical University of Catalonia (UPC), Barcelona East School of Engineering (EEBE), 08019 Barcelona, Spain
Barcelona Research Center in Multiscale Science and Engineering, UPC, EEBE, 08019, Barcelona, Spain
Institut de Recerca Sant Joan de Déu, 08034, Barcelona, Spain
Institute for Bioengineering of Catalonia (IBEC), 08028, Barcelona, Spain
Rupérez E:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Technical University of Catalonia (UPC), Barcelona East School of Engineering (EEBE), 08019 Barcelona, Spain
Barcelona Research Center in Multiscale Science and Engineering, UPC, EEBE, 08019, Barcelona, Spain
Institut de Recerca Sant Joan de Déu, 08034, Barcelona, Spain
Manero JM:
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Technical University of Catalonia (UPC), Barcelona East School of Engineering (EEBE), 08019 Barcelona, Spain
Barcelona Research Center in Multiscale Science and Engineering, UPC, EEBE, 08019, Barcelona, Spain
Institut de Recerca Sant Joan de Déu, 08034, Barcelona, Spain
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