Modulation of Serotonin-Related Genes by Extracellular Vesicles of the Probiotic Escherichia coli Nissle 1917 in the Interleukin-1ß-Induced Inflammation Model of Intestinal Epithelial Cells
Por:
Olivo-Martínez Y, Martínez-Ruiz S, Cordero-Alday C, Bosch M, Badia-Palacin J and Baldoma L
Publicada:
1 may 2024
Ahead of Print:
14 may 2024
Resumen:
Inflammatory bowel disease (IBD) is a chronic inflammatory condition involving dysregulated immune responses and imbalances in the gut microbiota in genetically susceptible individuals. Current therapies for IBD often have significant side-effects and limited success, prompting the search for novel therapeutic strategies. Microbiome-based approaches aim to restore the gut microbiota balance towards anti-inflammatory and mucosa-healing profiles. Extracellular vesicles (EVs) from beneficial gut microbes are emerging as potential postbiotics. Serotonin plays a crucial role in intestinal homeostasis, and its dysregulation is associated with IBD severity. Our study investigated the impact of EVs from the probiotic Nissle 1917 (EcN) and commensal E. coli on intestinal serotonin metabolism under inflammatory conditions using an IL-1 beta-induced inflammation model in Caco-2 cells. We found strain-specific effects. Specifically, EcN EVs reduced free serotonin levels by upregulating SERT expression through the downregulation of miR-24, miR-200a, TLR4, and NOD1. Additionally, EcN EVs mitigated IL-1 beta-induced changes in tight junction proteins and oxidative stress markers. These findings underscore the potential of postbiotic interventions as a therapeutic approach for IBD and related pathologies, with EcN EVs exhibiting promise in modulating serotonin metabolism and preserving intestinal barrier integrity. This study is the first to demonstrate the regulation of miR-24 and miR-200a by probiotic-derived EVs.
Filiaciones:
Olivo-Martínez Y:
Departament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, 08028 Barcelona, Spain
Biochemistry and Diseases Research Group, Facultad de Medicina, Universidad de Cartagena, Cartagena 130015, Colombia
Martínez-Ruiz S:
Departament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, 08028 Barcelona, Spain
Institut de Biomedicina de la Universitat de Barcelona (IBUB), 08028 Barcelona, Spain
Institut de Recerca Sant Joan de Déu (IRSJD), 08950 Barcelona, Spain
Cordero-Alday C:
Departament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, 08028 Barcelona, Spain
Institut de Biomedicina de la Universitat de Barcelona (IBUB), 08028 Barcelona, Spain
Institut de Recerca Sant Joan de Déu (IRSJD), 08950 Barcelona, Spain
Bosch M:
Unitat de Microscòpia Òptica Avançada, Centres Científics i Tecnològics, Universitat de Barcelona, 08028 Barcelona, Spain
Badia-Palacin J:
Departament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, 08028 Barcelona, Spain
Institut de Biomedicina de la Universitat de Barcelona (IBUB), 08028 Barcelona, Spain
Institut de Recerca Sant Joan de Déu (IRSJD), 08950 Barcelona, Spain
Baldoma L:
Departament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, 08028 Barcelona, Spain
Institut de Biomedicina de la Universitat de Barcelona (IBUB), 08028 Barcelona, Spain
Institut de Recerca Sant Joan de Déu (IRSJD), 08950 Barcelona, Spain
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