Injectable Plasma-Treated Alginate Hydrogel for Oxidative Stress Delivery to Induce Immunogenic Cell Death in Osteosarcoma
Por:
Zivanic, Milica, Espona-Noguera, Albert, Verswyvel, Hanne, Smits, Evelien, Bogaerts, Annemie, Lin, Abraham and Canal-Barnils C
Publicada:
1 abr 2024
Ahead of Print:
1 dic 2023
Resumen:
Cold atmospheric plasma (CAP) is a source of cell-damaging oxidant molecules that may be used as low-cost cancer treatment with minimal side effects. Liquids treated with cold plasma and enriched with oxidants are a modality for non-invasive treatment of internal tumors with cold plasma via injection. However, liquids are easily diluted with body fluids which impedes high and localized delivery of oxidants to the target. As an alternative, plasma-treated hydrogels (PTH) emerge as vehicles for the precise delivery of oxidants. This study reports an optimal protocol for the preparation of injectable alginate PTH that ensures the preservation of plasma-generated oxidants. The generation, storage, and release of oxidants from the PTH are assessed. The efficacy of the alginate PTH in cancer treatment is demonstrated in the context of cancer cell cytotoxicity and immunogenicity-release of danger signals and phagocytosis by immature dendritic cells, up to now unexplored for PTH. These are shown in osteosarcoma, a hard-to-treat cancer. The study aims to consolidate PTH as a novel cold plasma treatment modality for non-invasive or postoperative tumor treatment. The results offer a rationale for further exploration of alginate-based PTHs as a versatile platform in biomedical engineering.
A plasma-treated hydrogels (PTH) is proposed as a vehicle for the delivery of reactive oxygen and nitrogen species to internal tumors. Here, an injectable and shape-adaptable alginate PTH is reported for minimally invasive or post-operative cancer treatment. The PTH can release plasma-derived oxidants to kill osteosarcoma cells and induce the release of danger signals to promote their phagocytic uptake by immune cells.image
Filiaciones:
Univ Politecn Catalunya UPC, Biomat Biomech & Tissue Engn Grp, Dept Mat
Sci & Engn, Escola Engn Barcelona Est EEBE, C Eduard Maristany 14,
Barcelona 08019, Spain
Univ Politecn Catalunya UPC, Res Ctr Biomed Engn CREB, C Eduard
Maristany 14, Barcelona 08019, Spain
Inst Recerca Sant Joan de Deu, Biomat & Tissue Engn, Esplugues Del
Llobregat 08950, Santa Rosa, Spain
Univ Antwerp, Dept Chem, Plasma Lab Applicat Sustainabil & Med Antwerp
PLAS, Univ Pl 1, B-2610 Antwerp, Belgium
Univ Antwerp, Ctr Oncol Res CORE, Integrated Personalized & Precis Oncol
Network IPP, Univ Pl 1, B-2610 Antwerp, Belgium
Green Submitted, hybrid
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