New cases that expand the genotypic and phenotypic spectrum of Congenital NAD Deficiency Disorder.


Por: Szot JO, Slavotinek A, Chong K, Brandau O, Nezarati M, Cueto-González AM, Patel MS, Devine WP, Rego S, Acyinena AP, Shannon P, Myles-Reid D, Blaser S, Mieghem TV, Yavuz-Kienle H, Skladny H, Miller K, Riera MDT, Martínez SA, Tizzano E, Dupuis L, James Stavropoulos D, McNiven V, Mendoza-Londono R, Elliott AM, Phillips RS, Chapman G and Dunwoodie SL

Publicada: 1 jul 2021 Ahead of Print: 16 may 2021
Resumen:
Nicotinamide adenine dinucleotide (NAD) is an essential coenzyme involved in over 400 cellular reactions. During embryogenesis, mammals synthesize NAD de novo from dietary l -tryptophan via the kynurenine pathway. Biallelic, inactivating variants in three genes encoding enzymes of this biosynthesis pathway (KYNU, HAAO, and NADSYN1) disrupt NAD synthesis and have been identified in patients with multiple malformations of the heart, kidney, vertebrae, and limbs; these patients have Congenital NAD Deficiency Disorder HAAO and four families with biallelic variants in KYNU. These patients present similarly with multiple malformations of the heart, kidney, vertebrae, and limbs, of variable severity. We show that each variant identified in these patients results in loss-of-function, revealed by a significant reduction in NAD levels via yeast genetic complementation assays. For the first time, missense mutations are identified as a cause of malformation and shown to disrupt enzyme function. These missense and frameshift variants cause moderate to severe NAD deficiency in yeast, analogous to insufficient synthesized NAD in patients. We hereby expand the genotypic and corresponding phenotypic spectrum of Congenital NAD Deficiency Disorder.

Filiaciones:
Szot JO:
 Victor Chang Cardiac Research Institute, Sydney, New South Wales, Australia

Slavotinek A:
 Department of Pediatrics, University of California San Francisco, San Francisco, California, USA

Chong K:
 Prenatal Diagnosis and Medical Genetics Program, Department of Obstetrics and Gynecology, Mount Sinai Hospital, Toronto, Ontario, Canada

Brandau O:
 SYNLAB MVZ Humangenetik Mannheim, Mannheim, Germany

Nezarati M:
 Genetics Program, North York General Hospital, Toronto, Ontario, Canada

 Department of Paediatrics, University of Toronto, Toronto, Ontario, Canada

Cueto-González AM:
 Department of Clinical and Molecular Genetics, Vall d'Hebron Barcelona Hospital Campus, Barcelona, Spain

Patel MS:
 Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada

Devine WP:
 Department of Anatomic Pathology, University of California, San Francisco, California, USA

Rego S:
 Department of Pediatrics, University of California San Francisco, San Francisco, California, USA

Acyinena AP:
 Department of Pediatrics, University of California San Francisco, San Francisco, California, USA

Shannon P:
 Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario, Canada

Myles-Reid D:
 Prenatal Diagnosis and Medical Genetics Program, Department of Obstetrics and Gynecology, Mount Sinai Hospital, Toronto, Ontario, Canada

Blaser S:
 The Hospital for Sick Children, Toronto, Ontario, Canada

Mieghem TV:
 Fetal Medicine Program, Department of Obstetrics and Gynecology, Mount Sinai Hospital, Toronto, Ontario, Canada

Yavuz-Kienle H:
 SYNLAB MVZ Humangenetik Mannheim, Mannheim, Germany

Skladny H:
 SYNLAB MVZ Humangenetik Mannheim, Mannheim, Germany

Miller K:
 Genetics Program, North York General Hospital, Toronto, Ontario, Canada

Riera MDT:
 Metabolic Unit and Pediatric Neurology Department, Vall d'Hebron Barcelona Hospital Campus, Barcelona, Spain

Martínez SA:
 Fetal Medicine Unit and Obstetrics Department, Vall d'Hebron Barcelona Hospital Campus, Barcelona, Spain

Tizzano E:
 Department of Clinical and Molecular Genetics, Vall d'Hebron Barcelona Hospital Campus, Barcelona, Spain

 Medicine Genetics Group, Vall d'Hebron Research Institute, Vall d'Hebron Barcelona Hospital Campus, Autonomous University of Barcelona, Barcelona, Spain

Dupuis L:
 Department of Pediatrics, Division of Clinical and Metabolic Genetics, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada

James Stavropoulos D:
 Genome Diagnostics, Department of Paediatric Laboratory Medicine, The Hospital for Sick Children, Toronto, Ontario, Canada

McNiven V:
 Department of Pediatrics, Division of Clinical and Metabolic Genetics, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada

Mendoza-Londono R:
 Department of Pediatrics, Division of Clinical and Metabolic Genetics, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada

Elliott AM:
 Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada

CAUSES Study:
 Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada

Phillips RS:
 Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA

 Department of Chemistry, University of Georgia, Athens, Georgia, USA

Chapman G:
 Victor Chang Cardiac Research Institute, Sydney, New South Wales, Australia

 Faculty of Medicine, University of New South Wales, Sydney, New South Wales, Australia

Dunwoodie SL:
 Victor Chang Cardiac Research Institute, Sydney, New South Wales, Australia

 Faculty of Medicine, University of New South Wales, Sydney, New South Wales, Australia

 Faculty of Science, University of New South Wales, Sydney, New South Wales, Australia
ISSN: 10597794





HUMAN MUTATION
Editorial
WILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ, Estados Unidos America
Tipo de documento: Article
Volumen: 42 Número: 7
Páginas: 862-876
WOS Id: 000651009800001
ID de PubMed: 33942433
imagen Green Accepted

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