Testing of a novel automated point-of-care analyzer for blood ammonium monitoring in a clinical setting.
Por:
Rebollo-Calderón B, Calvo-López A, Ormazabal-Herrero A, Artuch-Iriberri R, Rosell-Ferrer X, Alonso-Chamarro J and Puyol M
Publicada:
1 jun 2025
Ahead of Print:
23 abr 2025
Resumen:
Certain diseases are marked by elevated ammonium levels in the blood, a condition known as hyperammonemia. Prompt detection and medical intervention are crucial to prevent potentially fatal outcomes. Therefore, ammonium levels should be monitored regularly, typically in referral hospitals where specialized and costly equipment is available. Although compact commercial devices are available for this purpose, none of them meet all the technical and analytical requirements needed for direct blood analysis, and current reported strategies have not been validated with enough samples to confirm results reliably. We present a robust and reliable automated point-of-care (POC) analyzer for the potentiometric determination of ammonium in blood. Comprising three computer-controlled modules-fluid management, detection, and data acquisition and transmission-this system combines portability, ease of use, and affordability. It can directly measure untreated blood samples, significantly reducing analysis time. Fully automated, it operates unsupervised with minimal lab personnel intervention. Analytical quality parameters include 5% RSD repeatability (n = 8), a limit of detection of 24 µM, a working range of 30-1000 µM and a sample volume of 215 µL. Successfully implemented in a hospital for 2 months, it analyzed 238 blood samples in parallel with the hospital's reference method showing comparable results (paired t-test, Passing-Bablok regression and Bland-Altman Plot) and randomly distributed errors, with a 4% accuracy calculated as mean error. Results indicate the POC analyzer effectiveness and reliability in a clinical setting compared to currently reported or commercially available equipment, being suitable for bedside monitoring of conditions associated with hyperammonemia in healthcare centers, including emergency rooms and clinics in developing countries.
Filiaciones:
Rebollo-Calderón B:
Group of Sensors and Biosensors, Department of Chemistry, Autonomous University of Barcelona, Edifici Cn, Bellaterra, Barcelona, 08193, Spain
:
Institut de Recerca Sant Joan de Déu, Passeig de Sant Joan de Déu, 2, Esplugues de Llobregat, Barcelona, 08950, Spain
Ormazabal-Herrero A:
Institut de Recerca Sant Joan de Déu, Passeig de Sant Joan de Déu, 2, Esplugues de Llobregat, Barcelona, 08950, Spain
Artuch-Iriberri R:
Institut de Recerca Sant Joan de Déu, Passeig de Sant Joan de Déu, 2, Esplugues de Llobregat, Barcelona, 08950, Spain
Rosell-Ferrer X:
Centre de Recerca en Enginyeria Biomèdica (CREB), Universitat Politécnica de Catalunya (UPC), Campus Diagonal Sud, Edifici H., Av. Diagonal 647, Barcelona, Spain
Alonso-Chamarro J:
Group of Sensors and Biosensors, Department of Chemistry, Autonomous University of Barcelona, Edifici Cn, Bellaterra, Barcelona, 08193, Spain
Puyol M:
Group of Sensors and Biosensors, Department of Chemistry, Autonomous University of Barcelona, Edifici Cn, Bellaterra, Barcelona, 08193, Spain
Green Submitted, Green Accepted, hybrid
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