Engineering Poly(lactic Acid)-Based Scaffolds for Abundant, Sustained, and Prolonged Lactate Release.
Por:
Haro Gutiérrez PA, Colombi S, Casanovas J, Resina L, Sans J, Engel E, Enshaei H, García-Torres JM, Pérez-Madrigal MM and Alemán C
Publicada:
11 jun 2025
Ahead of Print:
25 mar 2025
Resumen:
Recent studies have revealed that cardiac tissue regeneration is promoted by administering an initial dose of exogenous lactate and locally maintaining an abundant concentration of this compound for a prolonged period (i.e., around 10-14 days) through sustained release. The aim of this study is to develop a scaffold based on poly-(lactic acid) (PLA) for achieving a sustained daily release of lactate from the first day to the end of the recommended period. First, a five-layered electroresponsive scaffold has been engineered using three PLA layers (first, third, and fifth), each composed of electrospun microfibers (MFs), separated by spin coated lactate (second) and poly-(3,4-ethylenedioxythiophene):poly-(styrenesulfonate) (PEDOT:PSS) (fourth) intermediate layers. The hydrophobicity of the outer PLA layers (first and fifth) has been used to maintain the release of lactate from the intermediate second layer over 3 days, while the conducting fourth PEDOT:PSS layer has ensured a complete lactate release by electrostimulation. After that, in a second step, the same scaffold has been re-engineered to maintain the sustained release not only for a short period (3 days) but also for a prolonged period (>10 days). For this purpose, the PLA MFs of the intermediate third layer have been substituted by plasma-treated proteinase K-containing PLA MFs, obtained by electrospinning a PLA:enzyme mixture. The activity of the enzyme, which decomposes the ester bonds of PLA, combined with the effect of the plasma on the PLA structure, results in a prolonged sustained release that, in addition, can be modulated.
Filiaciones:
Haro Gutiérrez PA:
IMEM-BRT Group, Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/Eduard Maristany 10-14, Ed. I2, Barcelona 08019, Spain. RINGGOLD: 16767
Colombi S:
IMEM-BRT Group, Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/Eduard Maristany 10-14, Ed. I2, Barcelona 08019, Spain. RINGGOLD: 16767
Casanovas J:
Departament de Química, Física i Ciències Ambientals i del Sòl, Escola Politècnica Superior, Universitat de Lleida, c/Jaume II no. 69, Lleida E-25001, Spain. RINGGOLD: 16739
Resina L:
IMEM-BRT Group, Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/Eduard Maristany 10-14, Ed. I2, Barcelona 08019, Spain. RINGGOLD: 16767
Barcelona Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya, C/Eduard Maristany 10-14, Ed. C, Barcelona 08019, Spain. RINGGOLD: 16767
Sans J:
IMEM-BRT Group, Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/Eduard Maristany 10-14, Ed. I2, Barcelona 08019, Spain. RINGGOLD: 16767
Barcelona Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya, C/Eduard Maristany 10-14, Ed. C, Barcelona 08019, Spain. RINGGOLD: 16767
Engel E:
IMEM-BRT Group, Departament de Ciència i Enginyeria de Materials, EEBE, Universitat Politècnica de Catalunya (UPC), C/Eduard Maristany 10-14, Barcelona 08019, Spain
Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology, Baldiri Reixac 10-12, Barcelona 08028, Spain
CIBER en Bioingeniería, Biomateriales y Nanomedicina, CIBER-BBN, Zaragoza 50018, Spain
Enshaei H:
IMEM-BRT Group, Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/Eduard Maristany 10-14, Ed. I2, Barcelona 08019, Spain. RINGGOLD: 16767
Barcelona Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya, C/Eduard Maristany 10-14, Ed. C, Barcelona 08019, Spain. RINGGOLD: 16767
García-Torres JM:
Barcelona Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya, C/Eduard Maristany 10-14, Ed. C, Barcelona 08019, Spain. RINGGOLD: 16767
CIBER en Bioingeniería, Biomateriales y Nanomedicina, CIBER-BBN, Zaragoza 50018, Spain
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Universitat Politècnica de Catalunya (UPC), Barcelona 08019, Spain
Pérez-Madrigal MM:
IMEM-BRT Group, Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/Eduard Maristany 10-14, Ed. I2, Barcelona 08019, Spain. RINGGOLD: 16767
Barcelona Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya, C/Eduard Maristany 10-14, Ed. C, Barcelona 08019, Spain. RINGGOLD: 16767
Alemán C:
IMEM-BRT Group, Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/Eduard Maristany 10-14, Ed. I2, Barcelona 08019, Spain. RINGGOLD: 16767
Barcelona Research Center for Multiscale Science and Engineering, Universitat Politècnica de Catalunya, C/Eduard Maristany 10-14, Ed. C, Barcelona 08019, Spain. RINGGOLD: 16767
Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology, Baldiri Reixac 10-12, Barcelona 08028, Spain
Open Access
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