Cord Blood Exosomal miRNAs from Small-for-Gestational-Age Newborns: Association with Measures of Postnatal Catch-Up Growth and Insulin Resistance.
Por:
Díaz-Silva M, Quesada-López, T, Villarroya-Gombau F, López-Bermejo, A, de Zegher F, Ibañez-Toda L and Casano-Sancho P
Publicada:
15 jul 2025
Ahead of Print:
15 jul 2025
Resumen:
Small-for-gestational-age (SGA) infants who experience a marked postnatal catch-up, mainly in weight, are at risk for developing metabolic disorders; however, the underlying mechanisms are imprecise. Exosomes and their cargo (including miRNAs) mediate intercellular communication and may contribute to altered crosstalk among tissues. We assessed the miRNA profile in cord blood-derived exosomes from 10 appropriate-for-gestational-age (AGA) and 10 SGA infants by small RNA sequencing; differentially expressed miRNAs with a fold change =2.4 were validated by RT-qPCR in 40 AGA and 35 SGA infants and correlated with anthropometric, body composition (DXA) and endocrine-metabolic parameters at 4 and 12 mo. miR-1-3p, miR-133a-3p and miR-206 were down-regulated, whereas miR-372-3p, miR-519d-3p and miR-1299 were up-regulated in SGA infants. The target genes of these miRNAs related to insulin, RAP1, TGF beta and neurotrophin signaling. Receiver operating characteristic analysis disclosed that these miRNAs predicted with accuracy the 0-12 mo changes in body mass index and in total and abdominal fat and lean mass. In conclusion, the exosomal miRNA profile at birth differs between AGA and SGA infants and associates with measures of catch-up growth, insulin resistance and body composition through late infancy. Further follow-up of this population will disclose whether these associations persist into childhood, puberty and adolescence.
Filiaciones:
Díaz-Silva M:
Univ Barcelona, Inst Recerca St Joan Deu, Endocrinol Dept, Barcelona 08950, Spain
Inst Salud Carlos III, Ctr Invest Biomed Red Diabet & Enfermedades Metab, Madrid 28029, Spain
Quesada-López, T:
Inst Recerca Hosp St Creu & St Pau, Dept Biomed, Barcelona 08041, Spain
Hlth Inst Carlos III, Network Biomed Res Ctr Physiopathol Obes & Nutr CI, Madrid 28029, Spain
Villarroya-Gombau F:
Hlth Inst Carlos III, Network Biomed Res Ctr Physiopathol Obes & Nutr CI, Madrid 28029, Spain
Univ Barcelona, Biochem & Mol Biomed Dept, Inst Biomed, Barcelona 08028, Spain
Inst Recerca St Joan Deu, Esplugues 08950, Spain
López-Bermejo, A:
Univ Girona, Girona Inst Biomed Res IDIBGI, Fac Med, Pediat Endocrinol Res Grp, Girona 17190, Spain
Dr Josep Trueta Hosp, Girona 17190, Spain
de Zegher F:
Univ Leuven, Leuven Res & Dev, B-3000 Leuven, Belgium
Ibañez-Toda L:
Univ Barcelona, Inst Recerca St Joan Deu, Endocrinol Dept, Barcelona 08950, Spain
Inst Salud Carlos III, Ctr Invest Biomed Red Diabet & Enfermedades Metab, Madrid 28029, Spain
Casano-Sancho P:
Univ Barcelona, Inst Recerca St Joan Deu, Endocrinol Dept, Barcelona 08950, Spain
Inst Salud Carlos III, Ctr Invest Biomed Red Diabet & Enfermedades Metab, Madrid 28029, Spain
Green Accepted, Green Submitted, gold
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