Microbiological profile of slow-growing non-tuberculous mycobacteria species other than Mycobacterium avium complex.
Por:
Fernandez-Pittol, M, Batista, S, Narváez, S, Román, A, San Nicolás, L, Martínez, D, Oliver, L, González-Moreno, O, Martínez, JA, García, F, Amaro-Rodríguez, R, Soler, N, Gene-Giralt A, Gonzalez-Cuevas MA, Tudó, G and Gonzalez-Martin, J
Publicada:
25 abr 2025
Ahead of Print:
25 abr 2025
Resumen:
INTRODUCTION: Mycobaterium avium complex (MAC) and Mycobacterium abscessus complex are the primary agents of non-tuberculous mycobacteria infection. However, other species within the slow-growing group can also be potentially pathogenic, although information on these species is limited. OBJECTIVES: We conducted a prospective analysis of slow-growing species other than MAC, aimed at the identification and microbiological profiles of clinical samples from a tertiary hospital. The Microbiology Department of the Hospital Clinic of Barcelona, the Microbiology Laboratory of SYNLAB Laboratories, and the Microbiology Laboratory of Hospital Sant Joan de Deu participated in the study. METHODS: Species identification was conducted by MALDI-TOF MS and/or 16S rRNA and rpoB gene sequencing. Drug susceptibility tests (DST) were performed using the microdilution method. The results of the susceptibility profiles were compared with treatment guidelines, or the most recent literature related to each species. RESULTS: Twenty-five different species belonging to the slow-growing group were identified. The most frequently observed were M. xenopi, M. kansasii, M. gordonae, and M. marinum. In this series, M. lentiflavum presented the highest susceptibility profile, while M. simiae demonstrated the highest level of resistance. Clarithromycin, rifabutin, and amikacin demonstrated high levels of effectiveness across all species. The species most associated with infection, presented a high correlation with the clinical treatment guidelines. CONCLUSION: A specific susceptibility profile was observed among all the species. The in vitro profiles of the most frequent species correlated with the clinical treatment guidelines, reinforcing the supporting role of DST in the design of individualized treatment for each patient.
Filiaciones:
Fernandez-Pittol, M:
Hosp Clin Barcelona, Serv Microbiol, CDB, Barcelona, Spain
Univ Barcelona, Dept Fonaments Clin, Fac Med & Ciencies Salut, Barcelona, Spain
ISGLOBAL, Inst Global Hlth, Barcelona, Spain
Batista, S:
Univ Barcelona, Dept Fonaments Clin, Fac Med & Ciencies Salut, Barcelona, Spain
Inst Invest Biomed August Pi & Sunyer, Fundacio Recerca Clin Barcelona, Barcelona, Spain
Narváez, S:
Valles Occidental Parc Logist Salut Vial St Jordi, Catlab Ctr Analit Terrassa AIE, Serv Microbiol, Barcelona, Spain
Román, A:
Hosp Clin Barcelona, Serv Microbiol, CDB, Barcelona, Spain
San Nicolás, L:
Hosp Clin Barcelona, Serv Microbiol, CDB, Barcelona, Spain
Martínez, D:
Hosp Clin Barcelona, Serv Microbiol, CDB, Barcelona, Spain
Oliver, L:
SYNLAB Diagnost Globales, Dept Microbiol & Parasitol, Barcelona, Spain
González-Moreno, O:
SYNLAB Diagnost Globales, Dept Microbiol & Parasitol, Barcelona, Spain
Martínez, JA:
Univ Barcelona, Hosp Clin, Serv Malalties Infeccioses, Barcelona, Spain
Inst Salud Carlos III, CIBER Infect Dis CIBERINFEC, Madrid, Spain
García, F:
Univ Barcelona, Hosp Clin, Serv Malalties Infeccioses, Barcelona, Spain
Amaro-Rodríguez, R:
Univ Barcelona, Hosp Clin, Dept Pneumonol, Barcelona, Spain
Soler, N:
Univ Barcelona, Hosp Clin, Dept Pneumonol, Barcelona, Spain
Gene-Giralt A:
Hosp St Joan Deu, Barcelona, Spain
Gonzalez-Cuevas MA:
Hosp St Joan Deu, Barcelona, Spain
Tudó, G:
Hosp Clin Barcelona, Serv Microbiol, CDB, Barcelona, Spain
Univ Barcelona, Dept Fonaments Clin, Fac Med & Ciencies Salut, Barcelona, Spain
ISGLOBAL, Inst Global Hlth, Barcelona, Spain
Gonzalez-Martin, J:
Hosp Clin Barcelona, Serv Microbiol, CDB, Barcelona, Spain
Univ Barcelona, Dept Fonaments Clin, Fac Med & Ciencies Salut, Barcelona, Spain
ISGLOBAL, Inst Global Hlth, Barcelona, Spain
Inst Salud Carlos III, CIBER Infect Dis CIBERINFEC, Madrid, Spain
gold
|