Multiscale hierarchical surface structuring of zirconia using femtosecond laser and chemical etching: implications for cell response and antibacterial performance.
Por:
Garcia-de-Albeniz N, Müller DW, Mücklich F, Ginebra MP, Jiménez-Piqué E and Mas C
Publicada:
1 feb 2026
Ahead of Print:
8 dic 2025
Resumen:
This study investigates a novel strategy combining ultrashort pulsed-direct laser interference patterning (USP-DLIP) and chemical etching to create hierarchically micro- and nanorough topographies on zirconia with improved cell-instructive and antibacterial properties. Linear (L3) and grid (G3) micropatterns of 3 µm periodicity were fabricated via USP-DLIP, and subsequently treated with hydrofluoric acid to introduce an additional homogeneous nanotopography across the patterns. The individual and combined effects of micropatterning and etching on biological responses were evaluated using human mesenchymal stem cells (hMSCs) and two bacterial strains (Pseudomonas aeruginosa and Staphylococcus aureus) in mono- and co-culture settings. Micropatterns primarily guided cell morphology, alignment, and migration, while the introduced nanotopography enhanced focal adhesion formation and modulated cell-surface interactions. Antibacterial effects were found to be topography- and species-specific: micropatterns restricted P. aeruginosa colonization through a bacterial confinement mechanism, whereas etching-induced nanotopography effectively reduced S. aureus adhesion by limiting contact points. In co-culture assays, hMSC survival depended on a complex interplay between cell spreading and bacterial retention, dictated by surface features. Notably, chemical etching improved the antibacterial potential of the patterns against S. aureus, but it did not result in a synergistic improvement on cellular responses. Indeed, among all tested surfaces, the non-etched linear pattern (L3) consistently exhibited the most favorable outcomes -enhancing hMSC adhesion, migration, and osteogenic differentiation and mineralization, while reducing bacterial colonization and supporting cell survival under infection-like co-culture conditions.
Filiaciones:
Garcia-de-Albeniz N:
Center for Structural Integrity, Reliability and Micromechanics of Materials (CIEFMA), Department of Materials Science and Engineering, Universitat Politècnica de Catalunya. BarcelonaTech (UPC), Av. Eduard Maristany, 16, 08019, Barcelona, Spain
Biomaterials, Biomechanics and Tissue Engineering Group (BBT), Department of Materials Science and Engineering and Institute for Research and Innovation in Health (IRIS), Universitat Politècnica de Catalunya. BarcelonaTech (UPC), Av. Eduard Maristany, 16, 08019, Barcelona, Spain
Barcelona Research Center in Multiscale Science and Engineering, Universitat Politècnica de Catalunya. BarcelonaTech (UPC), Av. Eduard Maristany, 16, 08019, Barcelona, Spain
Müller DW:
Functional Materials, Department of Materials Science and Engineering, Saarland University, 66123, Saarbrücken, Germany
Mücklich F:
Functional Materials, Department of Materials Science and Engineering, Saarland University, 66123, Saarbrücken, Germany
Ginebra MP:
Biomaterials, Biomechanics and Tissue Engineering Group (BBT), Department of Materials Science and Engineering and Institute for Research and Innovation in Health (IRIS), Universitat Politècnica de Catalunya. BarcelonaTech (UPC), Av. Eduard Maristany, 16, 08019, Barcelona, Spain
Barcelona Research Center in Multiscale Science and Engineering, Universitat Politècnica de Catalunya. BarcelonaTech (UPC), Av. Eduard Maristany, 16, 08019, Barcelona, Spain
Centro de Investigación Biomédica en Red-Bioingeniería, Biomedicina y Nanomedicina (CIBER-BBN), Instituto de Salud Carlos III, Spain
Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology, 08028, Barcelona, Spain
Jiménez-Piqué E:
Center for Structural Integrity, Reliability and Micromechanics of Materials (CIEFMA), Department of Materials Science and Engineering, Universitat Politècnica de Catalunya. BarcelonaTech (UPC), Av. Eduard Maristany, 16, 08019, Barcelona, Spain
Barcelona Research Center in Multiscale Science and Engineering, Universitat Politècnica de Catalunya. BarcelonaTech (UPC), Av. Eduard Maristany, 16, 08019, Barcelona, Spain
Mas C:
Biomaterials, Biomechanics and Tissue Engineering Group (BBT), Department of Materials Science and Engineering and Institute for Research and Innovation in Health (IRIS), Universitat Politècnica de Catalunya. BarcelonaTech (UPC), Av. Eduard Maristany, 16, 08019, Barcelona, Spain
Barcelona Research Center in Multiscale Science and Engineering, Universitat Politècnica de Catalunya. BarcelonaTech (UPC), Av. Eduard Maristany, 16, 08019, Barcelona, Spain
Centro de Investigación Biomédica en Red-Bioingeniería, Biomedicina y Nanomedicina (CIBER-BBN), Instituto de Salud Carlos III, Spain
Open Access
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