A New Technique for Collection of Cerebrospinal Fluid in Rat Pups
Por:
Rodríguez-Fanjul J, Fernández-Feijóo CD and Camprubí-Camprubí M
Publicada:
1 ene 2015
Categoría:
Neuroscience (miscellaneous)
Resumen:
BACKGROUND: Neuroprotective strategies to prevent or decrease brain
injury in hypoxic ischemic newborns are one of the main research lines
in neonatology. Animal models have been used to assess the efficiency of
new therapeutic strategies. Brain damage biomarkers in cerebrospinal
fluid (CSF) are frequently used to evaluate the outcome at the bedside.
Despite the importance of this approach in clinical practice, there are
many difficulties in using it in small animals. The aim of this paper
was to describe a new technique for collecting CSF in rat pups.
Furthermore the reference values of S100beta protein levels, commonly
used in common clinical practice, were analyzed in animals between 7 to
12 days.
METHODS: 42 Wistar rat pups aged 7 to 12 days were used. CSF was
obtained by direct puncture of the cisterna magna with a 24-gauge
needle. S100beta protein levels were determined with enzyme-linked
immunosorbent assay (ELISA).
RESULTS: CSF was successfully obtained in 96% of the cases, with an
average amount of 21.28 mul (5-40 mul). Normal values for S100beta were
described. HI animals presented higher S100beta values than controls.
CONCLUSIONS: A simple, reproducible technique for CSF collection in rat
pups has been described. This new method will allow study of brain
injury biomarkers in newborn hypoxic ischemic animal models.
Filiaciones:
Rodríguez-Fanjul J:
Neonatal Intensive Care Unit Service, Hospital de Sant Joan de Déu Maternal, Fetal and Neonatology Center Barcelona (BCNatal), University of Barcelona, Barcelona, Spain
Fernández-Feijóo CD:
Neonatology Service, Complejo Hospitalario Universitario de Vigo, Galacia, Spain
Camprubí-Camprubí M:
Neonatal Intensive Care Unit Service, Hospital de Sant Joan de Déu Maternal, Fetal and Neonatology Center Barcelona (BCNatal), University of Barcelona, Barcelona, Spain
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